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10.1038/s41598-021-82833-w

http://scihub22266oqcxt.onion/10.1038/s41598-021-82833-w
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33558635!7870875!33558635
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suck abstract from ncbi


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pmid33558635      Sci+Rep 2021 ; 11 (1): 3318
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  • Potent neutralization of clinical isolates of SARS-CoV-2 D614 and G614 variants by a monomeric, sub-nanomolar affinity nanobody #MMPMID33558635
  • Valenzuela Nieto G; Jara R; Watterson D; Modhiran N; Amarilla AA; Himelreichs J; Khromykh AA; Salinas-Rebolledo C; Pinto T; Cheuquemilla Y; Margolles Y; Lopez Gonzalez Del Rey N; Miranda-Chacon Z; Cuevas A; Berking A; Deride C; Gonzalez-Moraga S; Mancilla H; Maturana D; Langer A; Toledo JP; Muller A; Uberti B; Krall P; Ehrenfeld P; Blesa J; Chana-Cuevas P; Rehren G; Schwefel D; Fernandez LA; Rojas-Fernandez A
  • Sci Rep 2021[Feb]; 11 (1): 3318 PMID33558635show ga
  • Despite unprecedented global efforts to rapidly develop SARS-CoV-2 treatments, in order to reduce the burden placed on health systems, the situation remains critical. Effective diagnosis, treatment, and prophylactic measures are urgently required to meet global demand: recombinant antibodies fulfill these requirements and have marked clinical potential. Here, we describe the fast-tracked development of an alpaca Nanobody specific for the receptor-binding-domain (RBD) of the SARS-CoV-2 Spike protein with potential therapeutic applicability. We present a rapid method for nanobody isolation that includes an optimized immunization regimen coupled with VHH library E. coli surface display, which allows single-step selection of Nanobodies using a simple density gradient centrifugation of the bacterial library. The selected single and monomeric Nanobody, W25, binds to the SARS-CoV-2 S RBD with sub-nanomolar affinity and efficiently competes with ACE-2 receptor binding. Furthermore, W25 potently neutralizes SARS-CoV-2 wild type and the D614G variant with IC50 values in the nanomolar range, demonstrating its potential as antiviral agent.
  • |Angiotensin-Converting Enzyme 2/immunology[MESH]
  • |Animals[MESH]
  • |Antibodies, Neutralizing/*immunology[MESH]
  • |Antibodies, Viral/*immunology[MESH]
  • |Antibody Affinity/*genetics[MESH]
  • |COVID-19/*immunology/virology[MESH]
  • |Camelids, New World/immunology[MESH]
  • |Escherichia coli/genetics/metabolism[MESH]
  • |Green Fluorescent Proteins/genetics[MESH]
  • |HeLa Cells[MESH]
  • |Humans[MESH]
  • |Immunization[MESH]
  • |Male[MESH]
  • |Neutralization Tests[MESH]
  • |Peptide Library[MESH]
  • |Protein Binding/genetics[MESH]
  • |SARS-CoV-2/chemistry/*immunology/isolation & purification[MESH]
  • |Single-Domain Antibodies/*immunology[MESH]
  • |Spike Glycoprotein, Coronavirus/genetics/*immunology[MESH]


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