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10.1038/s41467-021-21118-2 http://scihub22266oqcxt.onion/10.1038/s41467-021-21118-2 33558493!7870668!33558493     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 848 Nephropedia Template TP {{tp|p=33558493|t=2021. SARS-CoV-2 D614G spike mutation increases entry efficiency with enhanced ACE2-binding affinity |pdf=|usr=}}{{33558493}} gab.com Text SARS-CoV-2 D614G spike mutation increases entry efficiency with enhanced ACE2-binding affinity JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33558493 #FOAvip The causative agent of the COVID-19 pandemic, SARS-CoV-2, is steadily mutating during continuous transmission among humans. Such mutations can occur in the spike (S) protein that binds to the ACE2 receptor and is cleaved by TMPRSS2. However, whether S mutations affect SARS-CoV-2 cell entry remains unknown. Here, we show that naturally occurring S mutations can reduce or enhance cell entry via ACE2 and TMPRSS2. A SARS-CoV-2 S-pseudotyped lentivirus exhibits substantially lower entry than that of SARS-CoV S. Among S variants, the D614G mutant shows the highest cell entry, as supported by structural and binding analyses. Nevertheless, the D614G mutation does not affect neutralization by antisera against prototypic viruses. Taken together, we conclude that the D614G mutation increases cell entry by acquiring higher affinity to ACE2 while maintaining neutralization susceptibility. Based on these findings, further worldwide surveillance is required to understand SARS-CoV-2 transmissibility among humans. Twit Text FOAVip SARS-CoV-2 D614G spike mutation increases entry efficiency with enhanced ACE2-binding affinity ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33558493 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33558493 #FOAvip English Wikipedia <ref>{{Cite pmid|33558493}}</ref> SARS-CoV-2 D614G spike mutation increases entry efficiency with enhanced ACE2-binding affinity #MMPMID33558493 Ozono S; Zhang Y; Ode H; Sano K; Tan TS; Imai K; Miyoshi K; Kishigami S; Ueno T; Iwatani Y; Suzuki T; Tokunaga K Nat Commun 2021[Feb]; 12 (1): 848 PMID33558493show ga The causative agent of the COVID-19 pandemic, SARS-CoV-2, is steadily mutating during continuous transmission among humans. Such mutations can occur in the spike (S) protein that binds to the ACE2 receptor and is cleaved by TMPRSS2. However, whether S mutations affect SARS-CoV-2 cell entry remains unknown. Here, we show that naturally occurring S mutations can reduce or enhance cell entry via ACE2 and TMPRSS2. A SARS-CoV-2 S-pseudotyped lentivirus exhibits substantially lower entry than that of SARS-CoV S. Among S variants, the D614G mutant shows the highest cell entry, as supported by structural and binding analyses. Nevertheless, the D614G mutation does not affect neutralization by antisera against prototypic viruses. Taken together, we conclude that the D614G mutation increases cell entry by acquiring higher affinity to ACE2 while maintaining neutralization susceptibility. Based on these findings, further worldwide surveillance is required to understand SARS-CoV-2 transmissibility among humans. |*Mutation[MESH] |*Virus Internalization[MESH] |Angiotensin-Converting Enzyme 2/*metabolism[MESH] |Binding, Competitive[MESH] |COVID-19/epidemiology/*prevention & control/virology[MESH] |Humans[MESH] |Models, Molecular[MESH] |Pandemics[MESH] |Protein Binding[MESH] |Protein Domains[MESH] |Receptors, Virus/metabolism[MESH] |SARS-CoV-2/*genetics/physiology[MESH] |Serine Endopeptidases/metabolism[MESH]SARS-CoV-2 D614G spike mutation increases entry efficiency with enhanc(epmc).pdf DeepDyve Pubget Overpricing