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10.1016/j.dental.2021.01.015 http://scihub22266oqcxt.onion/10.1016/j.dental.2021.01.015 33551188!7846220!33551188     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Dent+Mater 2021 ; 37 (3): e95-e97 Nephropedia Template TP {{tp|p=33551188|t=2021. Fast and cost-effective screening for SARS-CoV-2 variants in a routine diagnostic setting |pdf=|usr=}}{{33551188}} gab.com Text Fast and cost-effective screening for SARS-CoV-2 variants in a routine diagnostic setting JO: Dent Mater http://www.kidney.de/pget.php?&tw=1&pmid=33551188 #FOAvip OBJECTIVE: The aim is to recommend a fast and cost-effective screening procedure for UK/SA SARS-CoV-2 variants in a routing diagnostic setting. METHODS: A rapid procedure using qPCR is described to provide clinicians with information about the two currently most prevalent variants (B1.1.7 and B1.351) that harbour receptor binding domain mutation N501Y. The N501Y specific assay only delivers an amplification signal if the Y501 variant is present. RESULTS: 436 samples initially screened positive for SARS-CoV-2 were randomly selected. Only one of these samples showed a fluorescence signal increase indicative for the Y501 variant. The remaining 435 samples had a melting peak at 54 degrees C indicating the N501 wildtype. SIGNIFICANCE: The screening of a broad population base can still be performed with the established test system. In case of a positive test for SARS-CoV-2 and corresponding clinical and anamnestic indications, a second qPCR for the mutation N501Y can follow and deliver the result to public health authorities and to the treating physician within a few hours. Twit Text FOAVip Fast and cost-effective screening for SARS-CoV-2 variants in a routine diagnostic setting ????Dent Mater http://www.kidney.de/pget.php?&tw=1&pmid=33551188 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33551188 #FOAvip English Wikipedia <ref>{{Cite pmid|33551188}}</ref> Fast and cost-effective screening for SARS-CoV-2 variants in a routine diagnostic setting #MMPMID33551188 Durner J; Burggraf S; Czibere L; Tehrani A; Watts DC; Becker M Dent Mater 2021[Mar]; 37 (3): e95-e97 PMID33551188show ga OBJECTIVE: The aim is to recommend a fast and cost-effective screening procedure for UK/SA SARS-CoV-2 variants in a routing diagnostic setting. METHODS: A rapid procedure using qPCR is described to provide clinicians with information about the two currently most prevalent variants (B1.1.7 and B1.351) that harbour receptor binding domain mutation N501Y. The N501Y specific assay only delivers an amplification signal if the Y501 variant is present. RESULTS: 436 samples initially screened positive for SARS-CoV-2 were randomly selected. Only one of these samples showed a fluorescence signal increase indicative for the Y501 variant. The remaining 435 samples had a melting peak at 54 degrees C indicating the N501 wildtype. SIGNIFICANCE: The screening of a broad population base can still be performed with the established test system. In case of a positive test for SARS-CoV-2 and corresponding clinical and anamnestic indications, a second qPCR for the mutation N501Y can follow and deliver the result to public health authorities and to the treating physician within a few hours. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |COVID-19 Testing[MESH] |Cost-Benefit Analysis[MESH]Fast and cost-effective screening for SARS-CoV-2 variants in a routine(epmc).pdf DeepDyve Pubget Overpricing