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10.1038/s41467-021-21121-7 http://scihub22266oqcxt.onion/10.1038/s41467-021-21121-7 33547323!7864991!33547323     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 802 Nephropedia Template TP {{tp|p=33547323|t=2021. Rapid electrochemical detection of coronavirus SARS-CoV-2 |pdf=|usr=}}{{33547323}} gab.com Text Rapid electrochemical detection of coronavirus SARS-CoV-2 JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33547323 #FOAvip Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/muL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19. Twit Text FOAVip Rapid electrochemical detection of coronavirus SARS-CoV-2 ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33547323 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33547323 #FOAvip English Wikipedia <ref>{{Cite pmid|33547323}}</ref> Rapid electrochemical detection of coronavirus SARS-CoV-2 #MMPMID33547323 Chaibun T; Puenpa J; Ngamdee T; Boonapatcharoen N; Athamanolap P; O'Mullane AP; Vongpunsawad S; Poovorawan Y; Lee SY; Lertanantawong B Nat Commun 2021[Feb]; 12 (1): 802 PMID33547323show ga Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/muL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19. |Biosensing Techniques/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |Electrochemical Techniques/*methods[MESH] |Humans[MESH] |Nucleic Acid Amplification Techniques/methods[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/genetics/*isolation & purification/physiology[MESH]Rapid electrochemical detection of coronavirus SARS-CoV-2 (epmc).pdf DeepDyve Pubget Overpricing