Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1038/s41598-021-82765-5 http://scihub22266oqcxt.onion/10.1038/s41598-021-82765-5 33542424!7862381!33542424     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 3122 Nephropedia Template TP {{tp|p=33542424|t=2021. Sample pooling as a strategy for community monitoring for SARS-CoV-2 |pdf=|usr=}}{{33542424}} gab.com Text Sample pooling as a strategy for community monitoring for SARS-CoV-2 JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33542424 #FOAvip Sample pooling strategy was intended to determine the optimal parameters for group testing of pooled specimens for the detection of SARS-CoV-2 and process them without significant loss of test usability. Standard molecular diagnostic laboratory equipment, and commercially available centrifugal filters, RNA isolation kits and SARS Cov2 PCR tests were used. The basic idea was to combine and concentrate several samples to the maximal volume, which can be extracted with the single extraction column. Out of 16 tested pools, 12 were positive with cycle threshold (Ct) values within 0.5 and 3.01 Ct of the original individual specimens. The analysis of 112 specimens determined that 12 pools were positive, followed by identification of 6 positive individual specimens among the 112 tested. This testing was accomplished with the use of 16 extractions/PCR tests, resulting in saving of 96 reactions but adding the 40 centrifugal filters. The present study demonstrated that pool testing could detect even up to a single positive sample with Ct value as high as 34. According to the standard protocols, reagents and equipment, this pooling method can be applied easily in current clinical testing laboratories. Twit Text FOAVip Sample pooling as a strategy for community monitoring for SARS-CoV-2 ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33542424 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33542424 #FOAvip English Wikipedia <ref>{{Cite pmid|33542424}}</ref> Sample pooling as a strategy for community monitoring for SARS-CoV-2 #MMPMID33542424 Sawicki R; Korona-Glowniak I; Boguszewska A; Stec A; Polz-Dacewicz M Sci Rep 2021[Feb]; 11 (1): 3122 PMID33542424show ga Sample pooling strategy was intended to determine the optimal parameters for group testing of pooled specimens for the detection of SARS-CoV-2 and process them without significant loss of test usability. Standard molecular diagnostic laboratory equipment, and commercially available centrifugal filters, RNA isolation kits and SARS Cov2 PCR tests were used. The basic idea was to combine and concentrate several samples to the maximal volume, which can be extracted with the single extraction column. Out of 16 tested pools, 12 were positive with cycle threshold (Ct) values within 0.5 and 3.01 Ct of the original individual specimens. The analysis of 112 specimens determined that 12 pools were positive, followed by identification of 6 positive individual specimens among the 112 tested. This testing was accomplished with the use of 16 extractions/PCR tests, resulting in saving of 96 reactions but adding the 40 centrifugal filters. The present study demonstrated that pool testing could detect even up to a single positive sample with Ct value as high as 34. According to the standard protocols, reagents and equipment, this pooling method can be applied easily in current clinical testing laboratories. |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Humans[MESH] |RNA, Viral/*isolation & purification[MESH] |SARS-CoV-2/genetics/isolation & purification[MESH] |Sensitivity and Specificity[MESH]Sample pooling as a strategy for community monitoring for SARS-CoV-2 (epmc).pdf DeepDyve Pubget Overpricing