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10.1038/s41598-021-82498-5 http://scihub22266oqcxt.onion/10.1038/s41598-021-82498-5 33542325!7862230!33542325     free     free     free Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 225.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 3125 Nephropedia Template TP {{tp|p=33542325|t=2021. DNA-launched RNA replicon vaccines induce potent anti-SARS-CoV-2 immune responses in mice |pdf=|usr=}}{{33542325}} gab.com Text DNA-launched RNA replicon vaccines induce potent anti-SARS-CoV-2 immune responses in mice JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33542325 #FOAvip The outbreak of the SARS-CoV-2 virus and its rapid spread into a global pandemic made the urgent development of scalable vaccines to prevent coronavirus disease (COVID-19) a global health and economic imperative. Here, we characterized and compared the immunogenicity of two alphavirus-based DNA-launched self-replicating (DREP) vaccine candidates encoding either SARS-CoV-2 spike glycoprotein (DREP-S) or a spike ectodomain trimer stabilized in prefusion conformation (DREP-S(ecto)). We observed that the two DREP constructs were immunogenic in mice inducing both binding and neutralizing antibodies as well as T cell responses. Interestingly, the DREP coding for the unmodified spike turned out to be more potent vaccine candidate, eliciting high titers of SARS-CoV-2 specific IgG antibodies that were able to efficiently neutralize pseudotyped virus after a single immunization. In addition, both DREP constructs were able to efficiently prime responses that could be boosted with a heterologous spike protein immunization. These data provide important novel insights into SARS-CoV-2 vaccine design using a rapid response DNA vaccine platform. Moreover, they encourage the use of mixed vaccine modalities as a strategy to combat SARS-CoV-2. Twit Text FOAVip DNA-launched RNA replicon vaccines induce potent anti-SARS-CoV-2 immune responses in mice ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33542325 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33542325 #FOAvip English Wikipedia <ref>{{Cite pmid|33542325}}</ref> DNA-launched RNA replicon vaccines induce potent anti-SARS-CoV-2 immune responses in mice #MMPMID33542325 Szurgot I; Hanke L; Sheward DJ; Vidakovics LP; Murrell B; McInerney GM; Liljestrom P Sci Rep 2021[Feb]; 11 (1): 3125 PMID33542325show ga The outbreak of the SARS-CoV-2 virus and its rapid spread into a global pandemic made the urgent development of scalable vaccines to prevent coronavirus disease (COVID-19) a global health and economic imperative. Here, we characterized and compared the immunogenicity of two alphavirus-based DNA-launched self-replicating (DREP) vaccine candidates encoding either SARS-CoV-2 spike glycoprotein (DREP-S) or a spike ectodomain trimer stabilized in prefusion conformation (DREP-S(ecto)). We observed that the two DREP constructs were immunogenic in mice inducing both binding and neutralizing antibodies as well as T cell responses. Interestingly, the DREP coding for the unmodified spike turned out to be more potent vaccine candidate, eliciting high titers of SARS-CoV-2 specific IgG antibodies that were able to efficiently neutralize pseudotyped virus after a single immunization. In addition, both DREP constructs were able to efficiently prime responses that could be boosted with a heterologous spike protein immunization. These data provide important novel insights into SARS-CoV-2 vaccine design using a rapid response DNA vaccine platform. Moreover, they encourage the use of mixed vaccine modalities as a strategy to combat SARS-CoV-2. |Animals[MESH] |COVID-19 Vaccines/*immunology[MESH] |COVID-19/*prevention & control[MESH] |Female[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Mice[MESH] |Mice, Inbred C57BL[MESH] |SARS-CoV-2/*immunology[MESH] |Spike Glycoprotein, Coronavirus/*immunology[MESH]DNA-launched RNA replicon vaccines induce potent anti-SARS-CoV-2 immun(epmc).pdf DeepDyve Pubget Overpricing