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10.1038/s41598-021-82191-7 http://scihub22266oqcxt.onion/10.1038/s41598-021-82191-7 33542252!7862273!33542252     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 3061 Nephropedia Template TP {{tp|p=33542252|t=2021. Sensitive mass spectrometric determination of kinin-kallikrein system peptides in light of COVID-19 |pdf=|usr=}}{{33542252}} gab.com Text Sensitive mass spectrometric determination of kinin-kallikrein system peptides in light of COVID-19 JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33542252 #FOAvip The outbreak of COVID-19 has raised interest in the kinin-kallikrein system. Viral blockade of the angiotensin-converting enzyme 2 impedes degradation of the active kinin des-Arg(9)-bradykinin, which thus increasingly activates bradykinin receptors known to promote inflammation, cough, and edema-symptoms that are commonly observed in COVID-19. However, lean and reliable investigation of the postulated alterations is currently hindered by non-specific peptide adsorption, lacking sensitivity, and cross-reactivity of applicable assays. Here, an LC-MS/MS method was established to determine the following kinins in respiratory lavage fluids: kallidin, bradykinin, des-Arg(10)-kallidin, des-Arg(9)-bradykinin, bradykinin 1-7, bradykinin 2-9 and bradykinin 1-5. This method was fully validated according to regulatory bioanalytical guidelines of the European Medicine Agency and the US Food and Drug Administration and has a broad calibration curve range (up to a factor of 10(3)), encompassing low quantification limits of 4.4-22.8 pg/mL (depending on the individual kinin). The application of the developed LC-MS/MS method to nasal lavage fluid allowed for the rapid (~ 2 h), comprehensive and low-volume (100 microL) determination of kinins. Hence, this novel assay may support current efforts to investigate the pathophysiology of COVID-19, but can also be extended to other diseases. Twit Text FOAVip Sensitive mass spectrometric determination of kinin-kallikrein system peptides in light of COVID-19 ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33542252 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33542252 #FOAvip English Wikipedia <ref>{{Cite pmid|33542252}}</ref> Sensitive mass spectrometric determination of kinin-kallikrein system peptides in light of COVID-19 #MMPMID33542252 Gangnus T; Burckhardt BB Sci Rep 2021[Feb]; 11 (1): 3061 PMID33542252show ga The outbreak of COVID-19 has raised interest in the kinin-kallikrein system. Viral blockade of the angiotensin-converting enzyme 2 impedes degradation of the active kinin des-Arg(9)-bradykinin, which thus increasingly activates bradykinin receptors known to promote inflammation, cough, and edema-symptoms that are commonly observed in COVID-19. However, lean and reliable investigation of the postulated alterations is currently hindered by non-specific peptide adsorption, lacking sensitivity, and cross-reactivity of applicable assays. Here, an LC-MS/MS method was established to determine the following kinins in respiratory lavage fluids: kallidin, bradykinin, des-Arg(10)-kallidin, des-Arg(9)-bradykinin, bradykinin 1-7, bradykinin 2-9 and bradykinin 1-5. This method was fully validated according to regulatory bioanalytical guidelines of the European Medicine Agency and the US Food and Drug Administration and has a broad calibration curve range (up to a factor of 10(3)), encompassing low quantification limits of 4.4-22.8 pg/mL (depending on the individual kinin). The application of the developed LC-MS/MS method to nasal lavage fluid allowed for the rapid (~ 2 h), comprehensive and low-volume (100 microL) determination of kinins. Hence, this novel assay may support current efforts to investigate the pathophysiology of COVID-19, but can also be extended to other diseases. |*Kallikrein-Kinin System[MESH] |Adult[MESH] |Bradykinin/*analysis[MESH] |COVID-19[MESH] |Chromatography, Liquid[MESH] |Female[MESH] |Healthy Volunteers[MESH] |Humans[MESH] |Male[MESH] |Nasal Lavage Fluid/*chemistry[MESH] |Tandem Mass Spectrometry[MESH]Sensitive mass spectrometric determination of kinin-kallikrein system (epmc).pdf DeepDyve Pubget Overpricing