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10.1371/journal.pone.0246544 http://scihub22266oqcxt.onion/10.1371/journal.pone.0246544 33539474!7861376!33539474     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2021 ; 16 (2): e0246544 Nephropedia Template TP {{tp|p=33539474|t=2021. Swab pooling: A new method for large-scale RT-qPCR screening of SARS-CoV-2 avoiding sample dilution |pdf=|usr=}}{{33539474}} gab.com Text Swab pooling: A new method for large-scale RT-qPCR screening of SARS-CoV-2 avoiding sample dilution JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33539474 #FOAvip To minimize sample dilution effect on SARS-CoV-2 pool testing, we assessed analytical and diagnostic performance of a new methodology, namely swab pooling. In this method, swabs are pooled at the time of collection, as opposed to pooling of equal volumes from individually collected samples. Paired analysis of pooled and individual samples from 613 patients revealed 94 positive individuals. Having individual testing as reference, no false-positives or false-negatives were observed for swab pooling. In additional 18,922 patients screened with swab pooling (1,344 pools), mean Cq differences between individual and pool samples ranged from 0.1 (Cr.I. -0.98 to 1.17) to 2.09 (Cr.I. 1.24 to 2.94). Overall, 19,535 asymptomatic patients were screened using 4,400 RT-qPCR assays. This corresponds to an increase of 4.4 times in laboratory capacity and a reduction of 77% in required tests. Therefore, swab pooling represents a major alternative for reliable and large-scale screening of SARS-CoV-2 in low prevalence populations. Twit Text FOAVip Swab pooling: A new method for large-scale RT-qPCR screening of SARS-CoV-2 avoiding sample dilution ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33539474 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33539474 #FOAvip English Wikipedia <ref>{{Cite pmid|33539474}}</ref> Swab pooling: A new method for large-scale RT-qPCR screening of SARS-CoV-2 avoiding sample dilution #MMPMID33539474 Christoff AP; Cruz GNF; Sereia AFR; Boberg DR; de Bastiani DC; Yamanaka LE; Fongaro G; Stoco PH; Bazzo ML; Grisard EC; Hernandes C; de Oliveira LFV PLoS One 2021[]; 16 (2): e0246544 PMID33539474show ga To minimize sample dilution effect on SARS-CoV-2 pool testing, we assessed analytical and diagnostic performance of a new methodology, namely swab pooling. In this method, swabs are pooled at the time of collection, as opposed to pooling of equal volumes from individually collected samples. Paired analysis of pooled and individual samples from 613 patients revealed 94 positive individuals. Having individual testing as reference, no false-positives or false-negatives were observed for swab pooling. In additional 18,922 patients screened with swab pooling (1,344 pools), mean Cq differences between individual and pool samples ranged from 0.1 (Cr.I. -0.98 to 1.17) to 2.09 (Cr.I. 1.24 to 2.94). Overall, 19,535 asymptomatic patients were screened using 4,400 RT-qPCR assays. This corresponds to an increase of 4.4 times in laboratory capacity and a reduction of 77% in required tests. Therefore, swab pooling represents a major alternative for reliable and large-scale screening of SARS-CoV-2 in low prevalence populations. |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |Humans[MESH] |Mass Screening/methods[MESH] |Nasopharynx/virology[MESH] |RNA, Viral/analysis/genetics[MESH] |Retrospective Studies[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH]Swab pooling: A new method for large-scale RT-qPCR screening of SARS-C(epmc).pdf DeepDyve Pubget Overpricing