Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1021/acs.biomac.0c01727 http://scihub22266oqcxt.onion/10.1021/acs.biomac.0c01727 33539086!ä!33539086 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biomacromolecules 2021 ; 22 (3): 1231-1243 Nephropedia Template TP {{tp|p=33539086|t=2021. Virus-Like Particles as Positive Controls for COVID-19 RT-LAMP Diagnostic Assays |pdf=|usr=}}{{33539086}} gab.com Text Virus-Like Particles as Positive Controls for COVID-19 RT-LAMP Diagnostic Assays JO: Biomacromolecules http://www.kidney.de/pget.php?&tw=1&pmid=33539086 #FOAvip Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a rapid and inexpensive isothermal alternative to the current gold standard reverse transcription quantitative polymerase chain reaction (RT-qPCR) for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, unlike RT-qPCR, there are no consensus detection regions or optimal RT-LAMP methods, and most protocols do not include internal controls to ensure reliability. Naked RNAs, plasmids, or even RNA from infectious COVID-19 patients have been used as external positive controls for RT-LAMP assays, but such reagents lack the stability required for full-process control. To overcome the lack of proper internal and external positive controls and the instability of the detection RNA, we developed virus-like particles (VLPs) using bacteriophage Qbeta and plant virus cowpea chlorotic mottle virus (CCMV) for the encapsidation of target RNA, namely a so-called SARS-CoV-2 LAMP detection module (SLDM). The target RNA is a truncated segment of the SARS-CoV-2 nucleocapsid (N) gene and human RNase P gene (internal control) as positive controls for RT-qPCR and RT-LAMP. Target RNAs stably encapsidated in Qbeta and CCMV VLPs were previously shown to function as full-process controls in RT-qPCR assays, and here we show that SLDMs can fulfill the same function for RT-LAMP and swab-to-test (direct RT-LAMP with heat lysis) assays. The SLDM was validated in a clinical setting, highlighting the promise of VLPs as positive controls for molecular assays. Twit Text FOAVip Virus-Like Particles as Positive Controls for COVID-19 RT-LAMP Diagnostic Assays ????Biomacromolecules http://www.kidney.de/pget.php?&tw=1&pmid=33539086 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33539086 #FOAvip English Wikipedia <ref>{{Cite pmid|33539086}}</ref> Virus-Like Particles as Positive Controls for COVID-19 RT-LAMP Diagnostic Assays #MMPMID33539086 Chan SK; Du P; Ignacio C; Mehta S; Newton IG; Steinmetz NF Biomacromolecules 2021[Mar]; 22 (3): 1231-1243 PMID33539086show ga Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a rapid and inexpensive isothermal alternative to the current gold standard reverse transcription quantitative polymerase chain reaction (RT-qPCR) for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, unlike RT-qPCR, there are no consensus detection regions or optimal RT-LAMP methods, and most protocols do not include internal controls to ensure reliability. Naked RNAs, plasmids, or even RNA from infectious COVID-19 patients have been used as external positive controls for RT-LAMP assays, but such reagents lack the stability required for full-process control. To overcome the lack of proper internal and external positive controls and the instability of the detection RNA, we developed virus-like particles (VLPs) using bacteriophage Qbeta and plant virus cowpea chlorotic mottle virus (CCMV) for the encapsidation of target RNA, namely a so-called SARS-CoV-2 LAMP detection module (SLDM). The target RNA is a truncated segment of the SARS-CoV-2 nucleocapsid (N) gene and human RNase P gene (internal control) as positive controls for RT-qPCR and RT-LAMP. Target RNAs stably encapsidated in Qbeta and CCMV VLPs were previously shown to function as full-process controls in RT-qPCR assays, and here we show that SLDMs can fulfill the same function for RT-LAMP and swab-to-test (direct RT-LAMP with heat lysis) assays. The SLDM was validated in a clinical setting, highlighting the promise of VLPs as positive controls for molecular assays. |*Bromovirus/chemistry/genetics[MESH] |*COVID-19/diagnosis/genetics[MESH] |COVID-19 Nucleic Acid Testing/*standards[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/*standards[MESH] |Nucleic Acid Amplification Techniques/*standards[MESH]Virus-Like Particles as Positive Controls for COVID-19 RT-LAMP Diagnos(epmc).pdf DeepDyve Pubget Overpricing