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10.1038/s41587-021-00822-w http://scihub22266oqcxt.onion/10.1038/s41587-021-00822-w 33536629!ä!33536629 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\33536629.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Biotechnol 2021 ; 39 (6): 717-726 Nephropedia Template TP {{tp|p=33536629|t=2021. Treatment of influenza and SARS-CoV-2 infections via mRNA-encoded Cas13a in rodents |pdf=|usr=}}{{33536629}} gab.com Text Treatment of influenza and SARS-CoV-2 infections via mRNA-encoded Cas13a in rodents JO: Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=33536629 #FOAvip Cas13a has been used to target RNA viruses in cell culture, but efficacy has not been demonstrated in animal models. In this study, we used messenger RNA (mRNA)-encoded Cas13a for mitigating influenza virus A and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in mice and hamsters, respectively. We designed CRISPR RNAs (crRNAs) specific for PB1 and highly conserved regions of PB2 of influenza virus, and against the replicase and nucleocapsid genes of SARS-CoV-2, and selected the crRNAs that reduced viral RNA levels most efficiently in cell culture. We delivered polymer-formulated Cas13a mRNA and the validated guides to the respiratory tract using a nebulizer. In mice, Cas13a degraded influenza RNA in lung tissue efficiently when delivered after infection, whereas in hamsters, Cas13a delivery reduced SARS-CoV-2 replication and reduced symptoms. Our findings suggest that Cas13a-mediated targeting of pathogenic viruses can mitigate respiratory infections. Twit Text FOAVip Treatment of influenza and SARS-CoV-2 infections via mRNA-encoded Cas13a in rodents ????Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=33536629 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33536629 #FOAvip English Wikipedia <ref>{{Cite pmid|33536629}}</ref> Treatment of influenza and SARS-CoV-2 infections via mRNA-encoded Cas13a in rodents #MMPMID33536629 Blanchard EL; Vanover D; Bawage SS; Tiwari PM; Rotolo L; Beyersdorf J; Peck HE; Bruno NC; Hincapie R; Michel F; Murray J; Sadhwani H; Vanderheyden B; Finn MG; Brinton MA; Lafontaine ER; Hogan RJ; Zurla C; Santangelo PJ Nat Biotechnol 2021[Jun]; 39 (6): 717-726 PMID33536629show ga Cas13a has been used to target RNA viruses in cell culture, but efficacy has not been demonstrated in animal models. In this study, we used messenger RNA (mRNA)-encoded Cas13a for mitigating influenza virus A and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection in mice and hamsters, respectively. We designed CRISPR RNAs (crRNAs) specific for PB1 and highly conserved regions of PB2 of influenza virus, and against the replicase and nucleocapsid genes of SARS-CoV-2, and selected the crRNAs that reduced viral RNA levels most efficiently in cell culture. We delivered polymer-formulated Cas13a mRNA and the validated guides to the respiratory tract using a nebulizer. In mice, Cas13a degraded influenza RNA in lung tissue efficiently when delivered after infection, whereas in hamsters, Cas13a delivery reduced SARS-CoV-2 replication and reduced symptoms. Our findings suggest that Cas13a-mediated targeting of pathogenic viruses can mitigate respiratory infections. |Animals[MESH] |COVID-19/genetics/*therapy/virology[MESH] |CRISPR-Cas Systems/genetics[MESH] |Cricetinae[MESH] |Disease Models, Animal[MESH] |Humans[MESH] |Influenza, Human/genetics/*therapy/virology[MESH] |Mice[MESH] |Orthomyxoviridae/drug effects/genetics/pathogenicity[MESH] |RNA, Messenger/genetics/*pharmacology[MESH] |RNA, Viral/genetics[MESH] |Respiratory System/drug effects/metabolism[MESH]Treatment of influenza and SARS-CoV-2 infections via mRNA-encoded Cas1(epmc).pdf DeepDyve Pubget Overpricing