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10.1126/sciadv.abe5984

http://scihub22266oqcxt.onion/10.1126/sciadv.abe5984
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33536218!7857687!33536218
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suck abstract from ncbi


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pmid33536218      Sci+Adv 2021 ; 7 (6): ä
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  • A diagnostic host response biosignature for COVID-19 from RNA profiling of nasal swabs and blood #MMPMID33536218
  • Ng DL; Granados AC; Santos YA; Servellita V; Goldgof GM; Meydan C; Sotomayor-Gonzalez A; Levine AG; Balcerek J; Han LM; Akagi N; Truong K; Neumann NM; Nguyen DN; Bapat SP; Cheng J; Martin CS; Federman S; Foox J; Gopez A; Li T; Chan R; Chu CS; Wabl CA; Gliwa AS; Reyes K; Pan CY; Guevara H; Wadford D; Miller S; Mason CE; Chiu CY
  • Sci Adv 2021[Feb]; 7 (6): ä PMID33536218show ga
  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes coronavirus disease-19 (COVID-19), has emerged as the cause of a global pandemic. We used RNA sequencing to analyze 286 nasopharyngeal (NP) swab and 53 whole-blood (WB) samples from 333 patients with COVID-19 and controls. Overall, a muted immune response was observed in COVID-19 relative to other infections (influenza, other seasonal coronaviruses, and bacterial sepsis), with paradoxical down-regulation of several key differentially expressed genes. Hospitalized patients and outpatients exhibited up-regulation of interferon-associated pathways, although heightened and more robust inflammatory responses were observed in hospitalized patients with more clinically severe illness. Two-layer machine learning-based host classifiers consisting of complete (>1000 genes), medium (<100), and small (<20) gene biomarker panels identified COVID-19 disease with 85.1-86.5% accuracy when benchmarked using an independent test set. SARS-CoV-2 infection has a distinct biosignature that differs between NP swabs and WB and can be leveraged for COVID-19 diagnosis.
  • |Area Under Curve[MESH]
  • |COVID-19/*diagnosis/metabolism/pathology/virology[MESH]
  • |Gene Library[MESH]
  • |Humans[MESH]
  • |Machine Learning[MESH]
  • |Nasopharynx/*virology[MESH]
  • |RNA, Viral/blood/*metabolism[MESH]
  • |ROC Curve[MESH]
  • |Reverse Transcriptase Polymerase Chain Reaction[MESH]
  • |SARS-CoV-2/*genetics/isolation & purification[MESH]
  • |Sensitivity and Specificity[MESH]


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