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10.1080/15476286.2021.1885188 http://scihub22266oqcxt.onion/10.1080/15476286.2021.1885188 33530819!8078525!33530819     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       RNA+Biol 2021 ; 18 (5): 688-695 Nephropedia Template TP {{tp|p=33530819|t=2021. Peripheral blood microRNAs and the COVID-19 patient: methodological considerations, technical challenges and practice points |pdf=|usr=}}{{33530819}} gab.com Text Peripheral blood microRNAs and the COVID-19 patient: methodological considerations, technical challenges and practice points JO: RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=33530819 #FOAvip The COVID-19 emergency pandemic resulting from infection with SARS-CoV-2 represents a major threat to public health worldwide. There is an urgent clinical demand for easily accessible tools to address weaknesses and gaps in the management of COVID-19 patients. In this context, transcriptomic profiling of liquid biopsies, especially microRNAs (miRNAs), has recently emerged as a robust source of potential clinical indicators for medical decision-making. Nevertheless, the analysis of the circulating miRNA signature and its translation to clinical practice requires strict control of a wide array of methodological details. In this review, we indicate the main methodological aspects that should be addressed when evaluating the circulating miRNA profiles in COVID-19 patients, from preanalytical and analytical variables to the experimental design, impact of confounding, analysis of the data and interpretation of the findings, among others. Additionally, we provide practice points to ensure the rigour and reproducibility of miRNA-based biomarker investigations of this condition.Abbreviations: ACE: angiotensin-converting enzyme; ARDS: acute respiratory distress syndrome; COVID-19: coronavirus disease 2019; ERDN: early Detection Research Network; LMWH: low molecular weight heparin; miRNA: microRNA; ncRNA: noncoding RNA; SARS-CoV-2: severe acute respiratory syndrome coronavirus-2; SOP: standard operating procedure. Twit Text FOAVip Peripheral blood microRNAs and the COVID-19 patient: methodological considerations, technical challenges and practice points ????RNA Biol http://www.kidney.de/pget.php?&tw=1&pmid=33530819 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33530819 #FOAvip English Wikipedia <ref>{{Cite pmid|33530819}}</ref> Peripheral blood microRNAs and the COVID-19 patient: methodological considerations, technical challenges and practice points #MMPMID33530819 Pinilla L; Benitez ID; Gonzalez J; Torres G; Barbe F; de Gonzalo-Calvo D RNA Biol 2021[May]; 18 (5): 688-695 PMID33530819show ga The COVID-19 emergency pandemic resulting from infection with SARS-CoV-2 represents a major threat to public health worldwide. There is an urgent clinical demand for easily accessible tools to address weaknesses and gaps in the management of COVID-19 patients. In this context, transcriptomic profiling of liquid biopsies, especially microRNAs (miRNAs), has recently emerged as a robust source of potential clinical indicators for medical decision-making. Nevertheless, the analysis of the circulating miRNA signature and its translation to clinical practice requires strict control of a wide array of methodological details. In this review, we indicate the main methodological aspects that should be addressed when evaluating the circulating miRNA profiles in COVID-19 patients, from preanalytical and analytical variables to the experimental design, impact of confounding, analysis of the data and interpretation of the findings, among others. Additionally, we provide practice points to ensure the rigour and reproducibility of miRNA-based biomarker investigations of this condition.Abbreviations: ACE: angiotensin-converting enzyme; ARDS: acute respiratory distress syndrome; COVID-19: coronavirus disease 2019; ERDN: early Detection Research Network; LMWH: low molecular weight heparin; miRNA: microRNA; ncRNA: noncoding RNA; SARS-CoV-2: severe acute respiratory syndrome coronavirus-2; SOP: standard operating procedure. |*SARS-CoV-2[MESH] |COVID-19/*blood/*genetics/virology[MESH] |Gene Expression Profiling/*methods/standards[MESH] |Genetic Markers[MESH] |Humans[MESH] |Liquid Biopsy/methods/standards[MESH] |MicroRNAs/*blood/*genetics/isolation & purification[MESH] |Pandemics[MESH]Peripheral blood microRNAs and the COVID-19 patient: methodological c(epmc).pdf DeepDyve Pubget Overpricing