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10.1016/j.ebiom.2021.103230 http://scihub22266oqcxt.onion/10.1016/j.ebiom.2021.103230 33530000!7847186!33530000     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       EBioMedicine 2021 ; 64 (ä): 103230 Nephropedia Template TP {{tp|p=33530000|t=2021. SARS-CoV-2 persistence is associated with antigen-specific CD8 T-cell responses |pdf=|usr=}}{{33530000}} gab.com Text SARS-CoV-2 persistence is associated with antigen-specific CD8 T-cell responses JO: EBioMedicine http://www.kidney.de/pget.php?&tw=1&pmid=33530000 #FOAvip BACKGROUND: Upon SARS-CoV-2 infection, most individuals develop neutralizing antibodies and T-cell immunity. However, some individuals reportedly remain SARS-CoV-2 PCR positive by pharyngeal swabs weeks after recovery. Whether viral RNA in these persistent carriers is contagious and stimulates SARS-CoV-2-specific immune responses is unknown. METHODS: This cohort study was conducted between April 3(rd)-July 9(th) 2020, recruiting COVID-19 recovered individuals that were symptom-free for at least 14 days. We collected serum for SARS-CoV-2-specific total Ig, IgA and IgM detection by ELISA, pharyngeal swabs (two time points) for ddPCR and PBMCs for anti-SARS-CoV-2 CD8 T-cell dextramer analyses. FINDINGS: We enrolled 203 post-symptomatic participants with a previous RT-PCR-verified SARS-CoV-2 infection. At time point 1, a median of 23 days (range 15-44) after recovery, 26 individuals (12?8%) were PCR positive. At time point 2, 90 days (median, range 85-105) after recovery, 5 (5?3%) were positive. There was no difference in SARS-CoV-2 antibody levels between the PCR negative and positive group. The persistent PCR positive group however, had SARS-CoV-2-specific CD8 T-cell responses of significantly increased breadth and magnitude. Assisted contact tracing among persistent PCR positive individuals revealed zero new COVID-19 diagnoses among 757 close contacts. INTERPRETATION: Persistent pharyngeal SARS-CoV-2 PCR positivity in post-symptomatic individuals is associated with elevated cellular immune responses and thus, the viral RNA may represent replicating virus. However, transmission to close contacts was not observed indicating that persistent PCR positive individuals are not contagious at the post-symptomatic stage of the infection. Twit Text FOAVip SARS-CoV-2 persistence is associated with antigen-specific CD8 T-cell responses ????EBioMedicine http://www.kidney.de/pget.php?&tw=1&pmid=33530000 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33530000 #FOAvip English Wikipedia <ref>{{Cite pmid|33530000}}</ref> SARS-CoV-2 persistence is associated with antigen-specific CD8 T-cell responses #MMPMID33530000 Vibholm LK; Nielsen SSF; Pahus MH; Frattari GS; Olesen R; Andersen R; Monrad I; Andersen AHF; Thomsen MM; Konrad CV; Andersen SD; Hojen JF; Gunst JD; Ostergaard L; Sogaard OS; Schleimann MH; Tolstrup M EBioMedicine 2021[Feb]; 64 (ä): 103230 PMID33530000show ga BACKGROUND: Upon SARS-CoV-2 infection, most individuals develop neutralizing antibodies and T-cell immunity. However, some individuals reportedly remain SARS-CoV-2 PCR positive by pharyngeal swabs weeks after recovery. Whether viral RNA in these persistent carriers is contagious and stimulates SARS-CoV-2-specific immune responses is unknown. METHODS: This cohort study was conducted between April 3(rd)-July 9(th) 2020, recruiting COVID-19 recovered individuals that were symptom-free for at least 14 days. We collected serum for SARS-CoV-2-specific total Ig, IgA and IgM detection by ELISA, pharyngeal swabs (two time points) for ddPCR and PBMCs for anti-SARS-CoV-2 CD8 T-cell dextramer analyses. FINDINGS: We enrolled 203 post-symptomatic participants with a previous RT-PCR-verified SARS-CoV-2 infection. At time point 1, a median of 23 days (range 15-44) after recovery, 26 individuals (12?8%) were PCR positive. At time point 2, 90 days (median, range 85-105) after recovery, 5 (5?3%) were positive. There was no difference in SARS-CoV-2 antibody levels between the PCR negative and positive group. The persistent PCR positive group however, had SARS-CoV-2-specific CD8 T-cell responses of significantly increased breadth and magnitude. Assisted contact tracing among persistent PCR positive individuals revealed zero new COVID-19 diagnoses among 757 close contacts. INTERPRETATION: Persistent pharyngeal SARS-CoV-2 PCR positivity in post-symptomatic individuals is associated with elevated cellular immune responses and thus, the viral RNA may represent replicating virus. However, transmission to close contacts was not observed indicating that persistent PCR positive individuals are not contagious at the post-symptomatic stage of the infection. |*COVID-19 Nucleic Acid Testing[MESH] |*Reverse Transcriptase Polymerase Chain Reaction[MESH] |Adult[MESH] |Aged[MESH] |Aged, 80 and over[MESH] |CD8-Positive T-Lymphocytes/*immunology/metabolism[MESH] |COVID-19/blood/*immunology[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |RNA, Viral/blood/*immunology[MESH]SARS-CoV-2 persistence is associated with antigen-specific CD8 T-cell (epmc).pdf DeepDyve Pubget Overpricing