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Automated molecular testing of saliva for SARS-CoV-2 detection #MMPMID33529938
Diagn Microbiol Infect Dis 2021[May]; 100 (1): 115324 PMID33529938show ga
With surging global demand for SARS-CoV-2 testing capacity, laboratories seek automated, high-throughput molecular solutions, particularly for specimens not requiring specialized collection devices or viral transport media. Saliva specimens submitted from patients under investigation for COVID-19 from March to July 2020 were processed in the laboratory with sterile phosphate-buffered saline in a 1:2 dilution and tested using manual extraction and a commercial assay for detection of the SARS-CoV-2 E gene (LightMix(R)) in comparison to the Roche cobas(R) SARS-CoV-2 Test on the cobas(R) 6800 instrument. 34.4% (22/64) of saliva samples were positive for SARS-CoV-2. Positive and negative concordance between the LightMix(R) and cobas(R) assays were 100%. The overall invalid rate for saliva on the cobas(R) 6800 (1/128, 0.78%) was similar to the baseline invalid rate observed for nasopharyngeal swabs/viral transport media. Saliva is a feasible specimen type for SARS-CoV-2 testing on the cobas(R) 6800 platform, with potential to improve turnaround time and enhance testing capacity.