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10.1038/s41467-020-20639-6 http://scihub22266oqcxt.onion/10.1038/s41467-020-20639-6 33526784!7851131!33526784     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 724 Nephropedia Template TP {{tp|p=33526784|t=2021. A glucose meter interface for point-of-care gene circuit-based diagnostics |pdf=|usr=}}{{33526784}} gab.com Text A glucose meter interface for point-of-care gene circuit-based diagnostics JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33526784 #FOAvip Recent advances in cell-free synthetic biology have given rise to gene circuit-based sensors with the potential to provide decentralized and low-cost molecular diagnostics. However, it remains a challenge to deliver this sensing capacity into the hands of users in a practical manner. Here, we leverage the glucose meter, one of the most widely available point-of-care sensing devices, to serve as a universal reader for these decentralized diagnostics. We describe a molecular translator that can convert the activation of conventional gene circuit-based sensors into a glucose output that can be read by off-the-shelf glucose meters. We show the development of new glucogenic reporter systems, multiplexed reporter outputs and detection of nucleic acid targets down to the low attomolar range. Using this glucose-meter interface, we demonstrate the detection of a small-molecule analyte; sample-to-result diagnostics for typhoid, paratyphoid A/B; and show the potential for pandemic response with nucleic acid sensors for SARS-CoV-2. Twit Text FOAVip A glucose meter interface for point-of-care gene circuit-based diagnostics ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=33526784 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33526784 #FOAvip English Wikipedia <ref>{{Cite pmid|33526784}}</ref> A glucose meter interface for point-of-care gene circuit-based diagnostics #MMPMID33526784 Amalfitano E; Karlikow M; Norouzi M; Jaenes K; Cicek S; Masum F; Sadat Mousavi P; Guo Y; Tang L; Sydor A; Ma D; Pearson JD; Trcka D; Pinette M; Ambagala A; Babiuk S; Pickering B; Wrana J; Bremner R; Mazzulli T; Sinton D; Brumell JH; Green AA; Pardee K Nat Commun 2021[Feb]; 12 (1): 724 PMID33526784show ga Recent advances in cell-free synthetic biology have given rise to gene circuit-based sensors with the potential to provide decentralized and low-cost molecular diagnostics. However, it remains a challenge to deliver this sensing capacity into the hands of users in a practical manner. Here, we leverage the glucose meter, one of the most widely available point-of-care sensing devices, to serve as a universal reader for these decentralized diagnostics. We describe a molecular translator that can convert the activation of conventional gene circuit-based sensors into a glucose output that can be read by off-the-shelf glucose meters. We show the development of new glucogenic reporter systems, multiplexed reporter outputs and detection of nucleic acid targets down to the low attomolar range. Using this glucose-meter interface, we demonstrate the detection of a small-molecule analyte; sample-to-result diagnostics for typhoid, paratyphoid A/B; and show the potential for pandemic response with nucleic acid sensors for SARS-CoV-2. |*Point-of-Care Systems[MESH] |*Point-of-Care Testing[MESH] |Biosensing Techniques/instrumentation/*methods[MESH] |COVID-19/diagnosis/epidemiology/virology[MESH] |Gene Regulatory Networks/*genetics[MESH] |Glucose/*analysis/metabolism[MESH] |Humans[MESH] |Nucleic Acids/*analysis/genetics[MESH] |Pandemics[MESH] |SARS-CoV-2/isolation & purification/physiology[MESH]A glucose meter interface for point-of-care gene circuit-based diagnos(epmc).pdf DeepDyve Pubget Overpricing