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10.1016/j.xcrm.2021.100204

http://scihub22266oqcxt.onion/10.1016/j.xcrm.2021.100204
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suck abstract from ncbi


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pmid33521695      Cell+Rep+Med 2021 ; 2 (2): 100204
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  • Comprehensive analysis of T cell immunodominance and immunoprevalence of SARS-CoV-2 epitopes in COVID-19 cases #MMPMID33521695
  • Tarke A; Sidney J; Kidd CK; Dan JM; Ramirez SI; Yu ED; Mateus J; da Silva Antunes R; Moore E; Rubiro P; Methot N; Phillips E; Mallal S; Frazier A; Rawlings SA; Greenbaum JA; Peters B; Smith DM; Crotty S; Weiskopf D; Grifoni A; Sette A
  • Cell Rep Med 2021[Feb]; 2 (2): 100204 PMID33521695show ga
  • T cells are involved in control of SARS-CoV-2 infection. To establish the patterns of immunodominance of different SARS-CoV-2 antigens and precisely measure virus-specific CD4(+) and CD8(+) T cells, we study epitope-specific T cell responses of 99 convalescent coronavirus disease 2019 (COVID-19) cases. The SARS-CoV-2 proteome is probed using 1,925 peptides spanning the entire genome, ensuring an unbiased coverage of human leukocyte antigen (HLA) alleles for class II responses. For HLA class I, we study an additional 5,600 predicted binding epitopes for 28 prominent HLA class I alleles, accounting for wide global coverage. We identify several hundred HLA-restricted SARS-CoV-2-derived epitopes. Distinct patterns of immunodominance are observed, which differ for CD4(+) T cells, CD8(+) T cells, and antibodies. The class I and class II epitopes are combined into epitope megapools to facilitate identification and quantification of SARS-CoV-2-specific CD4(+) and CD8(+) T cells.
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