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Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Engineering+(Beijing) 2020 ; 6 (10): 1130-1140 Nephropedia Template TP
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A High-Throughput, Multi-Index Isothermal Amplification Platform for Rapid Detection of 19 Types of Common Respiratory Viruses Including SARS-CoV-2 #MMPMID33520332
Xing W; Liu Y; Wang H; Li S; Lin Y; Chen L; Zhao Y; Chao S; Huang X; Ge S; Deng T; Zhao T; Li B; Wang H; Wang L; Song Y; Jin R; He J; Zhao X; Liu P; Li W; Cheng J
Engineering (Beijing) 2020[Oct]; 6 (10): 1130-1140 PMID33520332show ga
Fast and accurate diagnosis and the immediate isolation of patients infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are regarded as the most effective measures to restrain the coronavirus disease 2019 (COVID-19) pandemic. Here, we present a high-throughput, multi-index nucleic acid isothermal amplification analyzer (RTisochip-W) employing a centrifugal microfluidic chip to detect 19 common respiratory viruses, including SARS-CoV-2, from 16 samples in a single run within 90?min. The limits of detection of all the viruses analyzed by the RTisochip-W system were equal to or less than 50 copies.muL(-1), which is comparable to those of conventional reverse transcription polymerase chain reaction. We also demonstrate that the RTisochip-W system possesses the advantages of good repeatability, strong robustness, and high specificity. Finally, we analyzed 201 cases of preclinical samples, 14 cases of COVID-19-positive samples, 25 cases of clinically diagnosed samples, and 614 cases of clinical samples from patients or suspected patients with respiratory tract infections using the RTisochip-W system. The test results matched the referenced results well and reflected the epidemic characteristics of the respiratory infectious diseases. The coincidence rate of the RTisochip-W with the referenced kits was 98.15% for the detection of SARS-CoV-2. Based on these extensive trials, we believe that the RTisochip-W system provides a powerful platform for fighting the COVID-19 pandemic.