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Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Vaccine 2021 ; 39 (8): 1241-1247 Nephropedia Template TP
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Recombinant SARS-CoV-2 RBD with a built in T helper epitope induces strong neutralization antibody response #MMPMID33516600
Su QD; Zou YN; Yi Y; Shen LP; Ye XZ; Zhang Y; Wang H; Ke H; Song JD; Hu KP; Cheng BL; Qiu F; Yu PC; Zhou WT; Zhao R; Cao L; Dong GF; Bi SL; Wu GZ; Gao GF; Zheng J
Vaccine 2021[Feb]; 39 (8): 1241-1247 PMID33516600show ga
Without approved vaccines and specific treatments, COVID-19 is spreading around the world with above 26 million cases and approximately 864 thousand deaths until now. An efficacious and affordable vaccine is urgently needed. The Val308 - Gly548 of spike protein of SARS-CoV-2 linked with Gln830 - Glu843 of Tetanus toxoid (TT peptide) (designated as S1-4) and without TT peptide (designated as S1-5) were expressed and renatured. The antigenicity and immunogenicity of S1-4 were evaluated by Western Blotting (WB) in vitro and immune responses in mice, respectively. The protective efficiency was measured preliminarily by microneutralization assay (MN50). The soluble S1-4 and S1-5 protein was prepared to high homogeneity and purity. Adjuvanted with Alum, S1-4 protein stimulated a strong antibody response in immunized mice and caused a major Th2-type cellular immunity supplemented with Th1-type immunity. Furthermore, the immunized sera could protect the Vero E6 cells from SARS-CoV-2 infection with neutralizing antibody titer 256. Recombinant SARS-CoV-2 RBD with a built in T helper epitope could stimulate both strong humoral immunity supplemented with cellular immunity in mice, demonstrating that it could be a promising subunit vaccine candidate.