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10.1016/j.jviromet.2021.114083 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2021.114083 33515664!7839394!33515664     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 290 (ä): 114083 Nephropedia Template TP {{tp|p=33515664|t=2021. Rapid detection of SARS-CoV-2 by pulse-controlled amplification (PCA) |pdf=|usr=}}{{33515664}} gab.com Text Rapid detection of SARS-CoV-2 by pulse-controlled amplification (PCA) JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33515664 #FOAvip In the current pandemic of SARS-CoV-2, rapid identification of infected individuals is crucial for management and control of the outbreak. However, transport of samples, sample processing and RT-qPCR analysis in laboratories are time-consuming. Here we present a prototype of a novel nucleic acid-based test format - pulse controlled amplification - that allows detection of SARS-CoV-2 directly from up to eight swab samples simultaneously without the need for RNA extraction within 25 min with a sensitivity of 100 % for samples with a viral load of >/= 1.6 x 10e3 copies/mul This new principle might pave the way to rapid and sensitive point of care testing. Twit Text FOAVip Rapid detection of SARS-CoV-2 by pulse-controlled amplification (PCA) ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33515664 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33515664 #FOAvip English Wikipedia <ref>{{Cite pmid|33515664}}</ref> Rapid detection of SARS-CoV-2 by pulse-controlled amplification (PCA) #MMPMID33515664 Zwirglmaier K; Weyh M; Kruger C; Ehmann R; Muller K; Wolfel R; Stoecker K J Virol Methods 2021[Apr]; 290 (ä): 114083 PMID33515664show ga In the current pandemic of SARS-CoV-2, rapid identification of infected individuals is crucial for management and control of the outbreak. However, transport of samples, sample processing and RT-qPCR analysis in laboratories are time-consuming. Here we present a prototype of a novel nucleic acid-based test format - pulse controlled amplification - that allows detection of SARS-CoV-2 directly from up to eight swab samples simultaneously without the need for RNA extraction within 25 min with a sensitivity of 100 % for samples with a viral load of >/= 1.6 x 10e3 copies/mul This new principle might pave the way to rapid and sensitive point of care testing. |COVID-19 Nucleic Acid Testing/*methods/standards[MESH] |COVID-19/*diagnosis[MESH] |Humans[MESH] |Nucleic Acid Amplification Techniques/*methods/standards[MESH] |Point-of-Care Testing[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/genetics/*isolation & purification[MESH]Rapid detection of SARS-CoV-2 by pulse-controlled amplification (PCA) (epmc).pdf DeepDyve Pubget Overpricing