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10.1016/j.jviromet.2021.114087 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2021.114087 33515663!7840429!33515663     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 290 (ä): 114087 Nephropedia Template TP {{tp|p=33515663|t=2021. The effect of heat-treatment on SARS-CoV-2 viability and detection |pdf=|usr=}}{{33515663}} gab.com Text The effect of heat-treatment on SARS-CoV-2 viability and detection JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33515663 #FOAvip The development of safe diagnostic protocols for working with SARS-CoV-2 clinical samples at Biosafety Level 2 (BSL2) requires understanding of the effect of heat-treatment on SARS-CoV-2 viability and downstream RT-PCR sensitivity. In this study heating SARS-CoV-2/England/2/2020 to 56 degrees C and 60 degrees C for 15, 30 and 60 min reduced the virus titre by between 2.1 and 4.9 log(10) pfu/mL (as determined by plaque assay). Complete inactivation did not occur and there was significant variability between replicates. Viable virus was detected by plaque assay after heat-treatment at 80 degrees C for 15 or 30 min but not 60 or 90 min. After heat-treatment at 80 degrees C for 60 min infectious virus was only detected by more sensitive virus culture. No viable virus was detected after heating to 80 degrees C for 90 min or 95 degrees C for 1 or 5 min. RT-PCR sensitivity was not compromised by heating to 56 degrees C and 60 degrees C. However, RT-PCR sensitivity was reduced (>/=3 Ct value increase) after heating the virus to 80 degrees C for 30 min or longer, or 95 degrees C for 1 or 5 min. In summary we found that the efficacy of heat-inactivation varies greatly depending on temperature and duration. Local validation of heat-inactivation and its effects downstream is therefore essential for molecular testing. Twit Text FOAVip The effect of heat-treatment on SARS-CoV-2 viability and detection ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33515663 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33515663 #FOAvip English Wikipedia <ref>{{Cite pmid|33515663}}</ref> The effect of heat-treatment on SARS-CoV-2 viability and detection #MMPMID33515663 Burton J; Love H; Richards K; Burton C; Summers S; Pitman J; Easterbrook L; Davies K; Spencer P; Killip M; Cane P; Bruce C; Roberts ADG J Virol Methods 2021[Apr]; 290 (ä): 114087 PMID33515663show ga The development of safe diagnostic protocols for working with SARS-CoV-2 clinical samples at Biosafety Level 2 (BSL2) requires understanding of the effect of heat-treatment on SARS-CoV-2 viability and downstream RT-PCR sensitivity. In this study heating SARS-CoV-2/England/2/2020 to 56 degrees C and 60 degrees C for 15, 30 and 60 min reduced the virus titre by between 2.1 and 4.9 log(10) pfu/mL (as determined by plaque assay). Complete inactivation did not occur and there was significant variability between replicates. Viable virus was detected by plaque assay after heat-treatment at 80 degrees C for 15 or 30 min but not 60 or 90 min. After heat-treatment at 80 degrees C for 60 min infectious virus was only detected by more sensitive virus culture. No viable virus was detected after heating to 80 degrees C for 90 min or 95 degrees C for 1 or 5 min. RT-PCR sensitivity was not compromised by heating to 56 degrees C and 60 degrees C. However, RT-PCR sensitivity was reduced (>/=3 Ct value increase) after heating the virus to 80 degrees C for 30 min or longer, or 95 degrees C for 1 or 5 min. In summary we found that the efficacy of heat-inactivation varies greatly depending on temperature and duration. Local validation of heat-inactivation and its effects downstream is therefore essential for molecular testing. |*Virus Inactivation[MESH] |COVID-19 Nucleic Acid Testing[MESH] |COVID-19/diagnosis[MESH] |Hot Temperature[MESH] |Humans[MESH] |SARS-CoV-2/*isolation & purification/*physiology[MESH] |Sensitivity and Specificity[MESH]The effect of heat-treatment on SARS-CoV-2 viability and detection (epmc).pdf DeepDyve Pubget Overpricing