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10.1038/s42003-021-01649-6 http://scihub22266oqcxt.onion/10.1038/s42003-021-01649-6 33514825!7846565!33514825     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Commun+Biol 2021 ; 4 (1): 129 Nephropedia Template TP {{tp|p=33514825|t=2021. SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus |pdf=|usr=}}{{33514825}} gab.com Text SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus JO: Commun Biol http://www.kidney.de/pget.php?&tw=1&pmid=33514825 #FOAvip Development of antibody protection during SARS-CoV-2 infection is a pressing question for public health and for vaccine development. We developed highly sensitive SARS-CoV-2-specific antibody and neutralization assays. SARS-CoV-2 Spike protein or Nucleocapsid protein specific IgG antibodies at titers more than 1:100,000 were detectable in all PCR+ subjects (n = 115) and were absent in the negative controls. Other isotype antibodies (IgA, IgG1-4) were also detected. SARS-CoV-2 neutralization was determined in COVID-19 and convalescent plasma at up to 10,000-fold dilution, using Spike protein pseudotyped lentiviruses, which were also blocked by neutralizing antibodies (NAbs). Hospitalized patients had up to 3000-fold higher antibody and neutralization titers compared to outpatients or convalescent plasma donors. Interestingly, some COVID-19 patients also possessed NAbs against SARS-CoV Spike protein pseudovirus. Together these results demonstrate the high specificity and sensitivity of our assays, which may impact understanding the quality or duration of the antibody response during COVID-19 and in determining the effectiveness of potential vaccines. Twit Text FOAVip SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus ????Commun Biol http://www.kidney.de/pget.php?&tw=1&pmid=33514825 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33514825 #FOAvip English Wikipedia <ref>{{Cite pmid|33514825}}</ref> SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus #MMPMID33514825 Dogan M; Kozhaya L; Placek L; Gunter C; Yigit M; Hardy R; Plassmeyer M; Coatney P; Lillard K; Bukhari Z; Kleinberg M; Hayes C; Arditi M; Klapper E; Merin N; Liang BT; Gupta R; Alpan O; Unutmaz D Commun Biol 2021[Jan]; 4 (1): 129 PMID33514825show ga Development of antibody protection during SARS-CoV-2 infection is a pressing question for public health and for vaccine development. We developed highly sensitive SARS-CoV-2-specific antibody and neutralization assays. SARS-CoV-2 Spike protein or Nucleocapsid protein specific IgG antibodies at titers more than 1:100,000 were detectable in all PCR+ subjects (n = 115) and were absent in the negative controls. Other isotype antibodies (IgA, IgG1-4) were also detected. SARS-CoV-2 neutralization was determined in COVID-19 and convalescent plasma at up to 10,000-fold dilution, using Spike protein pseudotyped lentiviruses, which were also blocked by neutralizing antibodies (NAbs). Hospitalized patients had up to 3000-fold higher antibody and neutralization titers compared to outpatients or convalescent plasma donors. Interestingly, some COVID-19 patients also possessed NAbs against SARS-CoV Spike protein pseudovirus. Together these results demonstrate the high specificity and sensitivity of our assays, which may impact understanding the quality or duration of the antibody response during COVID-19 and in determining the effectiveness of potential vaccines. |Adult[MESH] |Angiotensin-Converting Enzyme 2/chemistry/immunology/metabolism[MESH] |Antibodies, Neutralizing/biosynthesis/*chemistry[MESH] |Antibodies, Viral/biosynthesis/*chemistry[MESH] |COVID-19/*diagnosis/immunology/virology[MESH] |Convalescence[MESH] |Coronavirus Nucleocapsid Proteins/*chemistry/immunology/metabolism[MESH] |Enzyme-Linked Immunosorbent Assay/methods[MESH] |Epitopes/chemistry/immunology/metabolism[MESH] |Female[MESH] |Genetic Vectors/chemistry/metabolism[MESH] |Humans[MESH] |Immune Sera/chemistry[MESH] |Immunity, Humoral[MESH] |Lentivirus/genetics/immunology[MESH] |Male[MESH] |Middle Aged[MESH] |Neutralization Tests[MESH] |Phosphoproteins/chemistry/immunology/metabolism[MESH] |Protein Binding[MESH] |Receptors, Virus/chemistry/immunology/metabolism[MESH] |SARS-CoV-2/drug effects/*immunology/pathogenicity[MESH] |Severity of Illness Index[MESH] |Spike Glycoprotein, Coronavirus/*chemistry/immunology/metabolism[MESH]SARS-CoV-2 specific antibody and neutralization assays reveal the wide(epmc).pdf DeepDyve Pubget Overpricing