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10.1186/s12951-021-00768-w http://scihub22266oqcxt.onion/10.1186/s12951-021-00768-w 33514385!7844813!33514385     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 269.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Nanobiotechnology 2021 ; 19 (1): 33 Nephropedia Template TP {{tp|p=33514385|t=2021. Development of multivalent nanobodies blocking SARS-CoV-2 infection by targeting RBD of spike protein |pdf=|usr=}}{{33514385}} gab.com Text Development of multivalent nanobodies blocking SARS-CoV-2 infection by targeting RBD of spike protein JO: J Nanobiotechnology http://www.kidney.de/pget.php?&tw=1&pmid=33514385 #FOAvip BACKGROUND: The outbreak and pandemic of coronavirus SARS-CoV-2 caused significant threaten to global public health and economic consequences. It is extremely urgent that global people must take actions to develop safe and effective preventions and therapeutics. Nanobodies, which are derived from single?chain camelid antibodies, had shown antiviral properties in various challenge viruses. In this study, multivalent nanobodies with high affinity blocking SARS-CoV-2 spike interaction with ACE2 protein were developed. RESULTS: Totally, four specific nanobodies against spike protein and its RBD domain were screened from a naive VHH library. Among them, Nb91-hFc and Nb3-hFc demonstrated antiviral activity by neutralizing spike pseudotyped viruses in vitro. Subsequently, multivalent nanobodies were constructed to improve the neutralizing capacity. As a result, heterodimer nanobody Nb91-Nb3-hFc exhibited the strongest RBD-binding affinity and neutralizing ability against SARS-CoV-2 pseudoviruses with an IC(50) value at approximately 1.54 nM. CONCLUSIONS: The present study indicated that naive VHH library could be used as a potential resource for rapid acquisition and exploitation of antiviral nanobodies. Heterodimer nanobody Nb91-Nb3-hFc may serve as a potential therapeutic agent for the treatment of COVID-19. Twit Text FOAVip Development of multivalent nanobodies blocking SARS-CoV-2 infection by targeting RBD of spike protein ????J Nanobiotechnology http://www.kidney.de/pget.php?&tw=1&pmid=33514385 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33514385 #FOAvip English Wikipedia <ref>{{Cite pmid|33514385}}</ref> Development of multivalent nanobodies blocking SARS-CoV-2 infection by targeting RBD of spike protein #MMPMID33514385 Lu Q; Zhang Z; Li H; Zhong K; Zhao Q; Wang Z; Wu Z; Yang D; Sun S; Yang N; Zheng M; Chen Q; Long C; Guo W; Yang H; Nie C; Tong A J Nanobiotechnology 2021[Jan]; 19 (1): 33 PMID33514385show ga BACKGROUND: The outbreak and pandemic of coronavirus SARS-CoV-2 caused significant threaten to global public health and economic consequences. It is extremely urgent that global people must take actions to develop safe and effective preventions and therapeutics. Nanobodies, which are derived from single?chain camelid antibodies, had shown antiviral properties in various challenge viruses. In this study, multivalent nanobodies with high affinity blocking SARS-CoV-2 spike interaction with ACE2 protein were developed. RESULTS: Totally, four specific nanobodies against spike protein and its RBD domain were screened from a naive VHH library. Among them, Nb91-hFc and Nb3-hFc demonstrated antiviral activity by neutralizing spike pseudotyped viruses in vitro. Subsequently, multivalent nanobodies were constructed to improve the neutralizing capacity. As a result, heterodimer nanobody Nb91-Nb3-hFc exhibited the strongest RBD-binding affinity and neutralizing ability against SARS-CoV-2 pseudoviruses with an IC(50) value at approximately 1.54 nM. CONCLUSIONS: The present study indicated that naive VHH library could be used as a potential resource for rapid acquisition and exploitation of antiviral nanobodies. Heterodimer nanobody Nb91-Nb3-hFc may serve as a potential therapeutic agent for the treatment of COVID-19. |Antibodies, Neutralizing/immunology[MESH] |Antibodies, Viral/immunology[MESH] |Binding Sites[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Neutralization Tests[MESH] |Protein Binding[MESH] |Protein Domains[MESH] |SARS-CoV-2/immunology[MESH] |Single-Domain Antibodies/*immunology[MESH]Development of multivalent nanobodies blocking SARS-CoV-2 infection by(epmc).pdf DeepDyve Pubget Overpricing