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10.3390/bios11020033

http://scihub22266oqcxt.onion/10.3390/bios11020033
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33513845!7911868!33513845
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suck abstract from ncbi


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pmid33513845      Biosensors+(Basel) 2021 ; 11 (2): ä
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  • High-Sensitivity High-Throughput Detection of Nucleic Acid Targets on Metasurface Fluorescence Biosensors #MMPMID33513845
  • Iwanaga M
  • Biosensors (Basel) 2021[Jan]; 11 (2): ä PMID33513845show ga
  • Worldwide infection disease due to SARS-CoV-2 is tremendously affecting our daily lives. High-throughput detection methods for nucleic acids are emergently desired. Here, we show high-sensitivity and high-throughput metasurface fluorescence biosensors that are applicable for nucleic acid targets. The all-dielectric metasurface biosensors comprise silicon-on-insulator nanorod array and have prominent electromagnetic resonances enhancing fluorescence emission. For proof-of-concept experiment on the metasurface biosensors, we have conducted fluorescence detection of single-strand oligoDNAs, which model the partial sequences of SARS-CoV-2 RNA indicated by national infection institutes, and succeeded in the high-throughput detection at low concentrations on the order of 100 amol/mL without any amplification technique. As a direct detection method, the metasurface fluorescence biosensors exhibit high performance.
  • |Biosensing Techniques/*methods[MESH]
  • |COVID-19 Nucleic Acid Testing/*methods[MESH]
  • |COVID-19/*diagnosis[MESH]
  • |High-Throughput Screening Assays/methods[MESH]
  • |Humans[MESH]
  • |Molecular Diagnostic Techniques/methods[MESH]
  • |Nucleic Acid Amplification Techniques/methods[MESH]
  • |RNA, Viral/analysis[MESH]
  • |SARS-CoV-2/*genetics[MESH]


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