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10.1016/j.scitotenv.2021.145274 http://scihub22266oqcxt.onion/10.1016/j.scitotenv.2021.145274 33513496!7825999!33513496     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Total+Environ 2021 ; 770 (ä): 145274 Nephropedia Template TP {{tp|p=33513496|t=2021. The detection and stability of the SARS-CoV-2 RNA biomarkers in wastewater influent in Helsinki, Finland |pdf=|usr=}}{{33513496}} gab.com Text The detection and stability of the SARS-CoV-2 RNA biomarkers in wastewater influent in Helsinki, Finland JO: Sci Total Environ http://www.kidney.de/pget.php?&tw=1&pmid=33513496 #FOAvip Wastewater-based surveillance of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is used to monitor the population-level prevalence of the COVID-19 disease. In many cases, due to lockdowns or analytical delays, the analysis of wastewater samples might only be possible after prolonged storage. In this study, the effect of storage conditions on the RNA copy numbers of the SARS-CoV-2 virus in wastewater influent was studied and compared to the persistence of norovirus over time at 4 degrees C, -20 degrees C, and -75 degrees C using the reverse-transcription quantitative PCR (RT-qPCR) assays E-Sarbeco, N2, and norovirus GII. For the first time in Finland, the presence of SARS-CoV-2 RNA was tested in 24 h composite influent wastewater samples collected from Viikinmaki wastewater treatment plant, Helsinki, Finland. The detected and quantified SARS-CoV-2 RNA copy numbers of the wastewater sample aliquots taken during 19-20 April 2020 and stored for 29, 64, and 84 days remained surprisingly stable. In the stored samples, the SARS betacoronavirus and SARS-CoV-2 copy numbers, but not the norovirus GII copy numbers, seemed slightly higher when analyzed from the pre-centrifuged pellet-that is, the particulate matter of the influent-as compared with the supernatant (i.e., water fraction) used for ultrafiltration, although the difference was not statistically significant. Furthermore, when wastewater was spiked with SARS-CoV-2, linear decay at 4 degrees C was observed on the first 28 days, while no decay was visible within 58 days at -20 degrees C or -75 degrees C. In conclusion, freezing temperatures should be used for storage when immediate SARS-CoV-2 RNA analysis from the wastewater influent is not possible. Analysis of the particulate matter of the sample, in addition to the water fraction, can improve the detection frequency. Twit Text FOAVip The detection and stability of the SARS-CoV-2 RNA biomarkers in wastewater influent in Helsinki, Finland ????Sci Total Environ http://www.kidney.de/pget.php?&tw=1&pmid=33513496 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33513496 #FOAvip English Wikipedia <ref>{{Cite pmid|33513496}}</ref> The detection and stability of the SARS-CoV-2 RNA biomarkers in wastewater influent in Helsinki, Finland #MMPMID33513496 Hokajarvi AM; Rytkonen A; Tiwari A; Kauppinen A; Oikarinen S; Lehto KM; Kankaanpaa A; Gunnar T; Al-Hello H; Blomqvist S; Miettinen IT; Savolainen-Kopra C; Pitkanen T Sci Total Environ 2021[May]; 770 (ä): 145274 PMID33513496show ga Wastewater-based surveillance of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is used to monitor the population-level prevalence of the COVID-19 disease. In many cases, due to lockdowns or analytical delays, the analysis of wastewater samples might only be possible after prolonged storage. In this study, the effect of storage conditions on the RNA copy numbers of the SARS-CoV-2 virus in wastewater influent was studied and compared to the persistence of norovirus over time at 4 degrees C, -20 degrees C, and -75 degrees C using the reverse-transcription quantitative PCR (RT-qPCR) assays E-Sarbeco, N2, and norovirus GII. For the first time in Finland, the presence of SARS-CoV-2 RNA was tested in 24 h composite influent wastewater samples collected from Viikinmaki wastewater treatment plant, Helsinki, Finland. The detected and quantified SARS-CoV-2 RNA copy numbers of the wastewater sample aliquots taken during 19-20 April 2020 and stored for 29, 64, and 84 days remained surprisingly stable. In the stored samples, the SARS betacoronavirus and SARS-CoV-2 copy numbers, but not the norovirus GII copy numbers, seemed slightly higher when analyzed from the pre-centrifuged pellet-that is, the particulate matter of the influent-as compared with the supernatant (i.e., water fraction) used for ultrafiltration, although the difference was not statistically significant. Furthermore, when wastewater was spiked with SARS-CoV-2, linear decay at 4 degrees C was observed on the first 28 days, while no decay was visible within 58 days at -20 degrees C or -75 degrees C. In conclusion, freezing temperatures should be used for storage when immediate SARS-CoV-2 RNA analysis from the wastewater influent is not possible. Analysis of the particulate matter of the sample, in addition to the water fraction, can improve the detection frequency. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |Biomarkers[MESH] |Communicable Disease Control[MESH] |Finland[MESH] |Humans[MESH] |RNA, Viral[MESH]The detection and stability of the SARS-CoV-2 RNA biomarkers in wastew(epmc).pdf DeepDyve Pubget Overpricing