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10.2144/btn-2020-0153 http://scihub22266oqcxt.onion/10.2144/btn-2020-0153 33512248!7931620!33512248     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biotechniques 2021 ; 70 (3): 149-159 Nephropedia Template TP {{tp|p=33512248|t=2021. A comparison of DNA/RNA extraction protocols for high-throughput sequencing of microbial communities |pdf=|usr=}}{{33512248}} gab.com Text A comparison of DNA/RNA extraction protocols for high-throughput sequencing of microbial communities JO: Biotechniques http://www.kidney.de/pget.php?&tw=1&pmid=33512248 #FOAvip One goal of microbial ecology researchers is to capture the maximum amount of information from all organisms in a sample. The recent COVID-19 pandemic, caused by the RNA virus SARS-CoV-2, has highlighted a gap in traditional DNA-based protocols, including the high-throughput methods the authors previously established as field standards. To enable simultaneous SARS-CoV-2 and microbial community profiling, the authors compared the relative performance of two total nucleic acid extraction protocols with the authors' previously benchmarked protocol. The authors included a diverse panel of environmental and host-associated sample types, including body sites commonly swabbed for COVID-19 testing. Here the authors present results comparing the cost, processing time, DNA and RNA yield, microbial community composition, limit of detection and well-to-well contamination between these protocols. Twit Text FOAVip A comparison of DNA/RNA extraction protocols for high-throughput sequencing of microbial communities ????Biotechniques http://www.kidney.de/pget.php?&tw=1&pmid=33512248 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33512248 #FOAvip English Wikipedia <ref>{{Cite pmid|33512248}}</ref> A comparison of DNA/RNA extraction protocols for high-throughput sequencing of microbial communities #MMPMID33512248 Shaffer JP; Marotz C; Belda-Ferre P; Martino C; Wandro S; Estaki M; Salido RA; Carpenter CS; Zaramela LS; Minich JJ; Bryant M; Sanders K; Fraraccio S; Ackermann G; Humphrey G; Swafford AD; Miller-Montgomery S; Knight R Biotechniques 2021[Mar]; 70 (3): 149-159 PMID33512248show ga One goal of microbial ecology researchers is to capture the maximum amount of information from all organisms in a sample. The recent COVID-19 pandemic, caused by the RNA virus SARS-CoV-2, has highlighted a gap in traditional DNA-based protocols, including the high-throughput methods the authors previously established as field standards. To enable simultaneous SARS-CoV-2 and microbial community profiling, the authors compared the relative performance of two total nucleic acid extraction protocols with the authors' previously benchmarked protocol. The authors included a diverse panel of environmental and host-associated sample types, including body sites commonly swabbed for COVID-19 testing. Here the authors present results comparing the cost, processing time, DNA and RNA yield, microbial community composition, limit of detection and well-to-well contamination between these protocols. |Animals[MESH] |Biodiversity[MESH] |Cats[MESH] |Chemical Fractionation/methods[MESH] |DNA, Viral/*isolation & purification[MESH] |Feces/microbiology/virology[MESH] |Female[MESH] |Fermented Foods/microbiology[MESH] |High-Throughput Nucleotide Sequencing/*methods[MESH] |Humans[MESH] |Limit of Detection[MESH] |Male[MESH] |Metagenomics/methods[MESH] |Mice[MESH] |Microbiota/*genetics[MESH] |RNA, Ribosomal, 16S/*isolation & purification[MESH] |SARS-CoV-2/*genetics[MESH] |Saliva/microbiology/virology[MESH]A comparison of DNA/RNA extraction protocols for high-throughput seque(epmc).pdf DeepDyve Pubget Overpricing