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10.1038/s41598-021-81487-y http://scihub22266oqcxt.onion/10.1038/s41598-021-81487-y 33510181!7844049!33510181     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 2402 Nephropedia Template TP {{tp|p=33510181|t=2021. Direct diagnostic testing of SARS-CoV-2 without the need for prior RNA extraction |pdf=|usr=}}{{33510181}} gab.com Text Direct diagnostic testing of SARS-CoV-2 without the need for prior RNA extraction JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33510181 #FOAvip The COVID-19 pandemic has resulted in an urgent need for a rapid, point of care diagnostic testing that could be rapidly scaled on a worldwide level. We developed and tested a highly sensitive and robust assay based on reverse transcription loop mediated isothermal amplification (RT-LAMP) that uses readily available reagents and a simple heat block using contrived spike-in and actual clinical samples. RT-LAMP testing on RNA-spiked samples showed a limit of detection (LoD) of 2.5 copies/mul of viral transport media. RT-LAMP testing directly on clinical nasopharyngeal swab samples in viral transport media had an 85% positive percentage agreement (PPA) (17/20), and 100% negative percentage agreement (NPV) and delivered results in 30 min. Our optimized RT-LAMP based testing method is a scalable system that is sufficiently sensitive and robust to test for SARS-CoV-2 directly on clinical nasopharyngeal swab samples in viral transport media in 30 min at the point of care without the need for specialized or proprietary equipment or reagents. This cost-effective and efficient one-step testing method can be readily available for COVID-19 testing world-wide, especially in resource poor settings. Twit Text FOAVip Direct diagnostic testing of SARS-CoV-2 without the need for prior RNA extraction ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33510181 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33510181 #FOAvip English Wikipedia <ref>{{Cite pmid|33510181}}</ref> Direct diagnostic testing of SARS-CoV-2 without the need for prior RNA extraction #MMPMID33510181 Wei S; Kohl E; Djandji A; Morgan S; Whittier S; Mansukhani M; Hod E; D'Alton M; Suh Y; Williams Z Sci Rep 2021[Jan]; 11 (1): 2402 PMID33510181show ga The COVID-19 pandemic has resulted in an urgent need for a rapid, point of care diagnostic testing that could be rapidly scaled on a worldwide level. We developed and tested a highly sensitive and robust assay based on reverse transcription loop mediated isothermal amplification (RT-LAMP) that uses readily available reagents and a simple heat block using contrived spike-in and actual clinical samples. RT-LAMP testing on RNA-spiked samples showed a limit of detection (LoD) of 2.5 copies/mul of viral transport media. RT-LAMP testing directly on clinical nasopharyngeal swab samples in viral transport media had an 85% positive percentage agreement (PPA) (17/20), and 100% negative percentage agreement (NPV) and delivered results in 30 min. Our optimized RT-LAMP based testing method is a scalable system that is sufficiently sensitive and robust to test for SARS-CoV-2 directly on clinical nasopharyngeal swab samples in viral transport media in 30 min at the point of care without the need for specialized or proprietary equipment or reagents. This cost-effective and efficient one-step testing method can be readily available for COVID-19 testing world-wide, especially in resource poor settings. |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |Clinical Laboratory Techniques/methods[MESH] |Diagnostic Techniques and Procedures[MESH] |Diagnostic Tests, Routine[MESH] |Humans[MESH] |Limit of Detection[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Nucleic Acid Amplification Techniques/*methods[MESH] |Point-of-Care Testing[MESH] |RNA, Viral/genetics/*isolation & purification[MESH] |Reverse Transcription[MESH] |SARS-CoV-2/chemistry/*genetics/metabolism[MESH]Direct diagnostic testing of SARS-CoV-2 without the need for prior RNA(epmc).pdf DeepDyve Pubget Overpricing