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10.4062/biomolther.2020.226

http://scihub22266oqcxt.onion/10.4062/biomolther.2020.226
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33504682!8094074!33504682
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suck abstract from ncbi


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pmid33504682      Biomol+Ther+(Seoul) 2021 ; 29 (3): 273-281
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  • Differential Signaling and Virus Production in Calu-3 Cells and Vero Cells upon SARS-CoV-2 Infection #MMPMID33504682
  • Park BK; Kim D; Park S; Maharjan S; Kim J; Choi JK; Akauliya M; Lee Y; Kwon HJ
  • Biomol Ther (Seoul) 2021[May]; 29 (3): 273-281 PMID33504682show ga
  • Severe acute respiratory syndrome CoV-2 (SARS-CoV-2) is responsible for the current coronavirus disease 2019 (COVID-19) pandemic. Signaling pathways that are essential for virus production have potential as therapeutic targets against COVID-19. In this study, we investigated cellular responses in two cell lines, Vero and Calu-3, upon SARS-CoV-2 infection and evaluated the effects of pathway-specific inhibitors on virus production. SARS-CoV-2 infection induced dephosphorylation of STAT1 and STAT3, high virus production, and apoptosis in Vero cells. However, in Calu-3 cells, SARS-CoV-2 infection induced long-lasting phosphorylation of STAT1 and STAT3, low virus production, and no prominent apoptosis. Inhibitors that target STAT3 phosphorylation and dimerization reduced SARS-CoV-2 production in Calu-3 cells, but not in Vero cells. These results suggest a necessity to evaluate cellular consequences upon SARS-CoV-2 infection using various model cell lines to find out more appropriate cells recapitulating relevant responses to SARS-CoV-2 infection in vitro.
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