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10.1038/s41598-021-81245-0 http://scihub22266oqcxt.onion/10.1038/s41598-021-81245-0 33500453!7838253!33500453     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 2224 Nephropedia Template TP {{tp|p=33500453|t=2021. A new SYBR Green real-time PCR to detect SARS-CoV-2 |pdf=|usr=}}{{33500453}} gab.com Text A new SYBR Green real-time PCR to detect SARS-CoV-2 JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33500453 #FOAvip Phylogenetic analysis has demonstrated that the etiologic agent of the 2020 pandemic outbreak is a betacoronavirus named SARS-CoV-2. For public health interventions, a diagnostic test with high sensitivity and specificity is required. The gold standard protocol for diagnosis by the Word Health Organization (WHO) is RT-PCR. To detect low viral loads and perform large-scale screening, a low-cost diagnostic test is necessary. Here, we developed a cost-effective test capable of detecting SARS-CoV-2. We validated an auxiliary protocol for molecular diagnosis with the SYBR Green RT-PCR methodology to successfully screen negative cases of SARS-CoV-2. Our results revealed a set of primers with high specificity and no homology with other viruses from the Coronovideae family or human respiratory tract pathogenic viruses, presenting with complementarity only for rhinoviruses/enteroviruses and Legionella spp. Optimization of the annealing temperature and polymerization time led to a high specificity in the PCR products. We have developed a more affordable and swift methodology for negative SARS-CoV-2 screening. This methodology can be applied on a large scale to soften panic and economic burden through guidance for isolation strategies. Twit Text FOAVip A new SYBR Green real-time PCR to detect SARS-CoV-2 ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33500453 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33500453 #FOAvip English Wikipedia <ref>{{Cite pmid|33500453}}</ref> A new SYBR Green real-time PCR to detect SARS-CoV-2 #MMPMID33500453 Marinowic DR; Zanirati G; Rodrigues FVF; Grahl MVC; Alcara AM; Machado DC; Da Costa JC Sci Rep 2021[Jan]; 11 (1): 2224 PMID33500453show ga Phylogenetic analysis has demonstrated that the etiologic agent of the 2020 pandemic outbreak is a betacoronavirus named SARS-CoV-2. For public health interventions, a diagnostic test with high sensitivity and specificity is required. The gold standard protocol for diagnosis by the Word Health Organization (WHO) is RT-PCR. To detect low viral loads and perform large-scale screening, a low-cost diagnostic test is necessary. Here, we developed a cost-effective test capable of detecting SARS-CoV-2. We validated an auxiliary protocol for molecular diagnosis with the SYBR Green RT-PCR methodology to successfully screen negative cases of SARS-CoV-2. Our results revealed a set of primers with high specificity and no homology with other viruses from the Coronovideae family or human respiratory tract pathogenic viruses, presenting with complementarity only for rhinoviruses/enteroviruses and Legionella spp. Optimization of the annealing temperature and polymerization time led to a high specificity in the PCR products. We have developed a more affordable and swift methodology for negative SARS-CoV-2 screening. This methodology can be applied on a large scale to soften panic and economic burden through guidance for isolation strategies. |*Organic Chemicals[MESH] |*SARS-CoV-2[MESH] |Benzothiazoles[MESH] |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*diagnosis[MESH] |DNA, Single-Stranded[MESH] |Diamines[MESH] |Enterovirus[MESH] |Genome, Viral[MESH] |Humans[MESH] |Phylogeny[MESH] |Polymerase Chain Reaction[MESH] |Quinolines[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH] |Rhinovirus[MESH] |Sensitivity and Specificity[MESH] |Temperature[MESH]A new SYBR Green real-time PCR to detect SARS-CoV-2 (epmc).pdf DeepDyve Pubget Overpricing