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10.1128/JCM.01295-20

http://scihub22266oqcxt.onion/10.1128/JCM.01295-20
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33500363!8092752!33500363
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suck abstract from ncbi


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pmid33500363      J+Clin+Microbiol 2021 ; 59 (4): ä
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  • Pooling of Nasopharyngeal Swab Samples To Overcome a Global Shortage of Real-Time Reverse Transcription-PCR COVID-19 Test Kits #MMPMID33500363
  • More S; Narayanan S; Patil G; Ghosh P; Pushparaj S; Cooper E; Ritchey J; Cheruvu VK; Kaul A; Ramachandran A
  • J Clin Microbiol 2021[Mar]; 59 (4): ä PMID33500363show ga
  • The global outbreak and rapid spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have created an urgent need for large-scale testing of populations. There is a demand for high-throughput testing protocols that can be used for efficient and rapid testing of clinical specimens. We evaluated a pooled PCR protocol for testing nasopharyngeal (NP) swabs using known positive/negative and untested clinical samples that were assigned to pools of 5 or 10. In total, 630 samples were used in this study. Individual positive samples with cycle threshold (C(T) ) values as high as 33 could be consistently detected when pooled with 4 negative samples (pool of 5), and individual positive samples with C(T) values up to 31 could be consistently detected when pooled with 9 negative samples (pool of 10). Pooling of up to 5 samples can be employed in laboratories for the diagnosis of COVID-19 for efficient utilization of resources, rapid screening of a greater number of people, and faster reporting of test results.
  • |*COVID-19[MESH]
  • |Humans[MESH]
  • |Nasopharynx[MESH]
  • |RNA, Viral/genetics[MESH]
  • |Reverse Transcription[MESH]
  • |SARS-CoV-2[MESH]


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