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10.3390/ijms22031017 http://scihub22266oqcxt.onion/10.3390/ijms22031017 33498408!7864175!33498408     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33498408&cmd=llinks): Failed to open stream: HTTP request failed! 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A Novel Approach to the Bioluminescent Detection of the SARS-CoV-2 ORF1ab Gene by Coupling Isothermal RNA Reverse Transcription Amplification with a Digital PCR Approach |pdf=|usr=}}{{33498408}} gab.com Text A Novel Approach to the Bioluminescent Detection of the SARS-CoV-2 ORF1ab Gene by Coupling Isothermal RNA Reverse Transcription Amplification with a Digital PCR Approach JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=33498408 #FOAvip The COVID-19 pandemic caused by the SARS-CoV-2 virus, which first emerged in December 2019, represents an ongoing global public health emergency. Here, we developed an improved and highly sensitive approach to SARS-CoV-2 detection via coupling bioluminescence in real-time (BART) and reverse-transcriptase loop-mediated amplification (RT-LAMP) protocols (RT-LAMP-BART) and was also compatible with a digital LAMP system (Rainsuit), which did not allow for real-time quantification but did, nonetheless, facilitate absolute quantification with a comparable detection limit of 10(4) copies/mL. Through improving RNA availability in samples to ensure the target RNA present in reaction, we additionally developed a simulated digital RT-LAMP approach using this same principle to enlarge the overall reaction volume and to achieve real-time detection with a limit of detection of 10 copies/mL, and with further improvements in the overall dynamic range of this assay system being achieved through additional optimization. Twit Text FOAVip A Novel Approach to the Bioluminescent Detection of the SARS-CoV-2 ORF1ab Gene by Coupling Isothermal RNA Reverse Transcription Amplification with a Digital PCR Approach ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=33498408 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33498408 #FOAvip English Wikipedia <ref>{{Cite pmid|33498408}}</ref> A Novel Approach to the Bioluminescent Detection of the SARS-CoV-2 ORF1ab Gene by Coupling Isothermal RNA Reverse Transcription Amplification with a Digital PCR Approach #MMPMID33498408 Fei Z; Wei R; Cheng C; Xiao P Int J Mol Sci 2021[Jan]; 22 (3): ä PMID33498408show ga The COVID-19 pandemic caused by the SARS-CoV-2 virus, which first emerged in December 2019, represents an ongoing global public health emergency. Here, we developed an improved and highly sensitive approach to SARS-CoV-2 detection via coupling bioluminescence in real-time (BART) and reverse-transcriptase loop-mediated amplification (RT-LAMP) protocols (RT-LAMP-BART) and was also compatible with a digital LAMP system (Rainsuit), which did not allow for real-time quantification but did, nonetheless, facilitate absolute quantification with a comparable detection limit of 10(4) copies/mL. Through improving RNA availability in samples to ensure the target RNA present in reaction, we additionally developed a simulated digital RT-LAMP approach using this same principle to enlarge the overall reaction volume and to achieve real-time detection with a limit of detection of 10 copies/mL, and with further improvements in the overall dynamic range of this assay system being achieved through additional optimization. |COVID-19/*virology[MESH] |Humans[MESH] |Limit of Detection[MESH] |Luminescent Measurements/methods[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Nucleic Acid Amplification Techniques/*methods[MESH] |Polyproteins/genetics[MESH] |RNA, Viral/analysis/*genetics[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH] |Reverse Transcription[MESH] |SARS-CoV-2/*genetics[MESH]A Novel Approach to the Bioluminescent Detection of the SARS-CoV-2 ORF(epmc).pdf DeepDyve Pubget Overpricing