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10.1016/j.xcrm.2021.100193 http://scihub22266oqcxt.onion/10.1016/j.xcrm.2021.100193 33495757!7816583!33495757     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Rep+Med 2021 ; 2 (2): 100193 Nephropedia Template TP {{tp|p=33495757|t=2021. Sensitive detection of total anti-Spike antibodies and isotype switching in asymptomatic and symptomatic individuals with COVID-19 |pdf=|usr=}}{{33495757}} gab.com Text Sensitive detection of total anti-Spike antibodies and isotype switching in asymptomatic and symptomatic individuals with COVID-19 JO: Cell Rep Med http://www.kidney.de/pget.php?&tw=1&pmid=33495757 #FOAvip Early detection of infection is crucial to limit the spread of coronavirus disease 2019 (COVID-19). Here we develop a flow cytometry-based assay to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein antibodies in individuals with COVID-19. The assay detects specific immunoglobulin M (IgM), IgA, and IgG in individuals with COVID-19 and also acquisition of all IgG subclasses, with IgG1 being the most dominant. The antibody response is significantly higher at a later stage of infection. Furthermore, asymptomatic individuals with COVID-19 also develop specific IgM, IgA, and IgG, with IgG1 being the most dominant subclass. Although the antibody levels are lower in asymptomatic infection, the assay is highly sensitive and detects 97% of asymptomatic infections. These findings demonstrate that the assay can be used for serological analysis of symptomatic and asymptomatic infections, which may otherwise remain undetected. Twit Text FOAVip Sensitive detection of total anti-Spike antibodies and isotype switching in asymptomatic and symptomatic individuals with COVID-19 ????Cell Rep Med http://www.kidney.de/pget.php?&tw=1&pmid=33495757 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33495757 #FOAvip English Wikipedia <ref>{{Cite pmid|33495757}}</ref> Sensitive detection of total anti-Spike antibodies and isotype switching in asymptomatic and symptomatic individuals with COVID-19 #MMPMID33495757 Goh YS; Chavatte JM; Lim Jieling A; Lee B; Hor PX; Amrun SN; Lee CY; Chee RS; Wang B; Lee CY; Ngoh EZX; Wang CI; Young BE; Tambyah PA; Kalimuddin S; Pada S; Tan SY; Sun LJ; Chen MI; Leo YS; Lye DC; Ng LFP; Lin RTP; Renia L Cell Rep Med 2021[Feb]; 2 (2): 100193 PMID33495757show ga Early detection of infection is crucial to limit the spread of coronavirus disease 2019 (COVID-19). Here we develop a flow cytometry-based assay to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) protein antibodies in individuals with COVID-19. The assay detects specific immunoglobulin M (IgM), IgA, and IgG in individuals with COVID-19 and also acquisition of all IgG subclasses, with IgG1 being the most dominant. The antibody response is significantly higher at a later stage of infection. Furthermore, asymptomatic individuals with COVID-19 also develop specific IgM, IgA, and IgG, with IgG1 being the most dominant subclass. Although the antibody levels are lower in asymptomatic infection, the assay is highly sensitive and detects 97% of asymptomatic infections. These findings demonstrate that the assay can be used for serological analysis of symptomatic and asymptomatic infections, which may otherwise remain undetected. |Antibodies, Viral/*blood/immunology[MESH] |Asymptomatic Diseases[MESH] |COVID-19/immunology/*pathology/virology[MESH] |Flow Cytometry[MESH] |Humans[MESH] |Immunoglobulin Class Switching/*physiology[MESH] |Immunoglobulin G/*blood/immunology[MESH] |Immunologic Tests/methods[MESH] |SARS-CoV-2/isolation & purification/*metabolism[MESH]Sensitive detection of total anti-Spike antibodies and isotype switchi(epmc).pdf DeepDyve Pubget Overpricing