Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText.
Kick-your-searchterm to multiple Engines kick-your-query now !>
A dictionary by aggregated review articles of nephrology, medicine and the life sciences
Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

suck abstract from ncbi


10.1371/journal.ppat.1009246

http://scihub22266oqcxt.onion/10.1371/journal.ppat.1009246
suck pdf from google scholar
33493182!7861537!33493182
unlimited free pdf from europmc33493182    free
PDF from PMC    free
html from PMC    free

suck abstract from ncbi


Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534

Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534
pmid33493182      PLoS+Pathog 2021 ; 17 (1): e1009246
Nephropedia Template TP

gab.com Text

Twit Text FOAVip

Twit Text #

English Wikipedia


  • Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusion #MMPMID33493182
  • Papa G; Mallery DL; Albecka A; Welch LG; Cattin-Ortola J; Luptak J; Paul D; McMahon HT; Goodfellow IG; Carter A; Munro S; James LC
  • PLoS Pathog 2021[Jan]; 17 (1): e1009246 PMID33493182show ga
  • Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) infects cells by binding to the host cell receptor ACE2 and undergoing virus-host membrane fusion. Fusion is triggered by the protease TMPRSS2, which processes the viral Spike (S) protein to reveal the fusion peptide. SARS-CoV-2 has evolved a multibasic site at the S1-S2 boundary, which is thought to be cleaved by furin in order to prime S protein for TMPRSS2 processing. Here we show that CRISPR-Cas9 knockout of furin reduces, but does not prevent, the production of infectious SARS-CoV-2 virus. Comparing S processing in furin knockout cells to multibasic site mutants reveals that while loss of furin substantially reduces S1-S2 cleavage it does not prevent it. SARS-CoV-2 S protein also mediates cell-cell fusion, potentially allowing virus to spread virion-independently. We show that loss of furin in either donor or acceptor cells reduces, but does not prevent, TMPRSS2-dependent cell-cell fusion, unlike mutation of the multibasic site that completely prevents syncytia formation. Our results show that while furin promotes both SARS-CoV-2 infectivity and cell-cell spread it is not essential, suggesting furin inhibitors may reduce but not abolish viral spread.
  • |*Cell Fusion[MESH]
  • |*Virus Internalization[MESH]
  • |Animals[MESH]
  • |COVID-19[MESH]
  • |CRISPR-Cas Systems[MESH]
  • |Chlorocebus aethiops[MESH]
  • |Furin/*genetics[MESH]
  • |Gene Knockout Techniques[MESH]
  • |HEK293 Cells[MESH]
  • |Humans[MESH]
  • |Protein Structure, Tertiary[MESH]
  • |SARS-CoV-2[MESH]
  • |Serine Endopeptidases[MESH]
  • |Spike Glycoprotein, Coronavirus/*chemistry[MESH]


  • DeepDyve
  • Pubget Overpricing
  • suck abstract from ncbi

    Linkout box