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10.1371/journal.ppat.1009246 http://scihub22266oqcxt.onion/10.1371/journal.ppat.1009246 33493182!7861537!33493182     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33493182&cmd=llinks): Failed to open stream: HTTP request failed! 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Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusion |pdf=|usr=}}{{33493182}} gab.com Text Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusion JO: PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=33493182 #FOAvip Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) infects cells by binding to the host cell receptor ACE2 and undergoing virus-host membrane fusion. Fusion is triggered by the protease TMPRSS2, which processes the viral Spike (S) protein to reveal the fusion peptide. SARS-CoV-2 has evolved a multibasic site at the S1-S2 boundary, which is thought to be cleaved by furin in order to prime S protein for TMPRSS2 processing. Here we show that CRISPR-Cas9 knockout of furin reduces, but does not prevent, the production of infectious SARS-CoV-2 virus. Comparing S processing in furin knockout cells to multibasic site mutants reveals that while loss of furin substantially reduces S1-S2 cleavage it does not prevent it. SARS-CoV-2 S protein also mediates cell-cell fusion, potentially allowing virus to spread virion-independently. We show that loss of furin in either donor or acceptor cells reduces, but does not prevent, TMPRSS2-dependent cell-cell fusion, unlike mutation of the multibasic site that completely prevents syncytia formation. Our results show that while furin promotes both SARS-CoV-2 infectivity and cell-cell spread it is not essential, suggesting furin inhibitors may reduce but not abolish viral spread. Twit Text FOAVip Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusion ????PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=33493182 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33493182 #FOAvip English Wikipedia <ref>{{Cite pmid|33493182}}</ref> Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for infection and cell-cell fusion #MMPMID33493182 Papa G; Mallery DL; Albecka A; Welch LG; Cattin-Ortola J; Luptak J; Paul D; McMahon HT; Goodfellow IG; Carter A; Munro S; James LC PLoS Pathog 2021[Jan]; 17 (1): e1009246 PMID33493182show ga Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) infects cells by binding to the host cell receptor ACE2 and undergoing virus-host membrane fusion. Fusion is triggered by the protease TMPRSS2, which processes the viral Spike (S) protein to reveal the fusion peptide. SARS-CoV-2 has evolved a multibasic site at the S1-S2 boundary, which is thought to be cleaved by furin in order to prime S protein for TMPRSS2 processing. Here we show that CRISPR-Cas9 knockout of furin reduces, but does not prevent, the production of infectious SARS-CoV-2 virus. Comparing S processing in furin knockout cells to multibasic site mutants reveals that while loss of furin substantially reduces S1-S2 cleavage it does not prevent it. SARS-CoV-2 S protein also mediates cell-cell fusion, potentially allowing virus to spread virion-independently. We show that loss of furin in either donor or acceptor cells reduces, but does not prevent, TMPRSS2-dependent cell-cell fusion, unlike mutation of the multibasic site that completely prevents syncytia formation. Our results show that while furin promotes both SARS-CoV-2 infectivity and cell-cell spread it is not essential, suggesting furin inhibitors may reduce but not abolish viral spread. |*Cell Fusion[MESH] |*Virus Internalization[MESH] |Animals[MESH] |COVID-19[MESH] |CRISPR-Cas Systems[MESH] |Chlorocebus aethiops[MESH] |Furin/*genetics[MESH] |Gene Knockout Techniques[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Protein Structure, Tertiary[MESH] |SARS-CoV-2[MESH] |Serine Endopeptidases[MESH] |Spike Glycoprotein, Coronavirus/*chemistry[MESH]Furin cleavage of SARS-CoV-2 Spike promotes but is not essential for i(epmc).pdf DeepDyve Pubget Overpricing