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10.1016/j.bios.2021.113005 http://scihub22266oqcxt.onion/10.1016/j.bios.2021.113005 33486135!7813512!33486135     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron 2021 ; 177 (ä): 113005 Nephropedia Template TP {{tp|p=33486135|t=2021. A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores |pdf=|usr=}}{{33486135}} gab.com Text A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores JO: Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33486135 #FOAvip The coronavirus disease 2019 (COVID-19) pandemic has been a major public health challenge in 2020. Early diagnosis of COVID-19 is the most effective method to control disease spread and prevent further mortality. As such, a high-precision and rapid yet economic assay method is urgently required. Herein, we propose an innovative method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using isothermal amplification of nucleic acids on a mesh containing multiple microfluidic pores. Hybridization of pathogen DNA and immobilized probes forms a DNA hydrogel by rolling circle amplification and, consequently, blocks the pores to prevent fluid movement, as observed. Following optimization of several factors, including pore size, mesh location, and precision microfluidics, the limit of detection (LOD) for SARS-CoV-2 was determined to be 0.7 aM at 15-min incubation. These results indicate rapid, easy, and effective detection with a moderate-sized LOD of the target pathogen by remote point-of-care testing and without the requirement of any sophisticated device. Twit Text FOAVip A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores ????Biosens Bioelectron http://www.kidney.de/pget.php?&tw=1&pmid=33486135 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33486135 #FOAvip English Wikipedia <ref>{{Cite pmid|33486135}}</ref> A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microfluidic pores #MMPMID33486135 Kim HS; Abbas N; Shin S Biosens Bioelectron 2021[Apr]; 177 (ä): 113005 PMID33486135show ga The coronavirus disease 2019 (COVID-19) pandemic has been a major public health challenge in 2020. Early diagnosis of COVID-19 is the most effective method to control disease spread and prevent further mortality. As such, a high-precision and rapid yet economic assay method is urgently required. Herein, we propose an innovative method to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using isothermal amplification of nucleic acids on a mesh containing multiple microfluidic pores. Hybridization of pathogen DNA and immobilized probes forms a DNA hydrogel by rolling circle amplification and, consequently, blocks the pores to prevent fluid movement, as observed. Following optimization of several factors, including pore size, mesh location, and precision microfluidics, the limit of detection (LOD) for SARS-CoV-2 was determined to be 0.7 aM at 15-min incubation. These results indicate rapid, easy, and effective detection with a moderate-sized LOD of the target pathogen by remote point-of-care testing and without the requirement of any sophisticated device. |*Point-of-Care Testing[MESH] |Biosensing Techniques/economics/instrumentation/methods[MESH] |COVID-19 Nucleic Acid Testing/economics/instrumentation/*methods[MESH] |COVID-19/*diagnosis/virology[MESH] |DNA Probes/chemistry/genetics[MESH] |Equipment Design[MESH] |Humans[MESH] |Hydrogels/*chemistry[MESH] |Immobilized Nucleic Acids/*chemistry/genetics[MESH] |Lab-On-A-Chip Devices[MESH] |Limit of Detection[MESH] |Molecular Diagnostic Techniques/economics/instrumentation/methods[MESH] |Nucleic Acid Amplification Techniques/economics/instrumentation/methods[MESH]A rapid diagnosis of SARS-CoV-2 using DNA hydrogel formation on microf(epmc).pdf DeepDyve Pubget Overpricing