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10.1016/j.cca.2021.01.011 http://scihub22266oqcxt.onion/10.1016/j.cca.2021.01.011 33485902!7826022!33485902     free     free     free       Clin+Chim+Acta 2021 ; 516 (ä): 1-7 Nephropedia Template TP {{tp|p=33485902|t=2021. rRT-PCR for SARS-CoV-2: Analytical considerations |pdf=|usr=}}{{33485902}} gab.com Text rRT-PCR for SARS-CoV-2: Analytical considerations JO: Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=33485902 #FOAvip The COVID-19 pandemic remains a significant problem involving health systems worldwide. Accurate and early detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection is critical for minimizing spread and initiating treatment. Among test methods, real-time reverse-transcriptase polymerase-chain-reaction (rRT-PCR) is considered the gold standard. Although this test has high specificity and relatively high sensitivity, the occurrence of falsely negative results in symptomatic patients and/or having a positive CT scan remains a challenge. Sources of error can be pre-analytical (sampling, storage and processing), analytical (RNA extraction, cDNA synthesis and amplification) and post-analytical (interpretation and analysis and test reporting). These potential sources of error and efforts to mitigate are reviewed in this article with an emphasis on the analytical phase. Twit Text FOAVip rRT-PCR for SARS-CoV-2: Analytical considerations ????Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=33485902 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33485902 #FOAvip English Wikipedia <ref>{{Cite pmid|33485902}}</ref> rRT-PCR for SARS-CoV-2: Analytical considerations #MMPMID33485902 Rahbari R; Moradi N; Abdi M Clin Chim Acta 2021[May]; 516 (ä): 1-7 PMID33485902show ga The COVID-19 pandemic remains a significant problem involving health systems worldwide. Accurate and early detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection is critical for minimizing spread and initiating treatment. Among test methods, real-time reverse-transcriptase polymerase-chain-reaction (rRT-PCR) is considered the gold standard. Although this test has high specificity and relatively high sensitivity, the occurrence of falsely negative results in symptomatic patients and/or having a positive CT scan remains a challenge. Sources of error can be pre-analytical (sampling, storage and processing), analytical (RNA extraction, cDNA synthesis and amplification) and post-analytical (interpretation and analysis and test reporting). These potential sources of error and efforts to mitigate are reviewed in this article with an emphasis on the analytical phase. |Humans[MESH] |Research Design[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH]rRT-PCR for SARS-CoV-2: Analytical considerations (epmc).pdf DeepDyve Pubget Overpricing