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10.1016/j.aca.2020.12.014 http://scihub22266oqcxt.onion/10.1016/j.aca.2020.12.014 33485583!7997732!33485583     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Anal+Chim+Acta 2021 ; 1147 (ä): 30-37 Nephropedia Template TP {{tp|p=33485583|t=2021. 3D-printed electrode as a new platform for electrochemical immunosensors for virus detection |pdf=|usr=}}{{33485583}} gab.com Text 3D-printed electrode as a new platform for electrochemical immunosensors for virus detection JO: Anal Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=33485583 #FOAvip Simple, low-cost, and sensitive new platforms for electrochemical immunosensors for virus detection have been attracted attention due to the recent pandemic caused by a new type of coronavirus (SARS-CoV-2). In the present work, we report for the first time the construction of an immunosensor using a commercial 3D conductive filament of carbon black and polylactic acid (PLA) to detect Hantavirus Araucaria nucleoprotein (Np) as a proof-of-concept. The recognition biomolecule was anchored directly at the filament surface by using N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-Hydroxysuccinimide (EDC/NHS). Conductive and non-conductive composites of PLA were characterized using thermal gravimetric analysis (TGA), revealing around 30% w/w of carbon in the filament. Morphological features of composites were obtained from SEM and TEM measurements. FTIR measurement revealed that crosslinking agents were covalently bonded at the filament surface. Electrochemical techniques such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used for the evaluation of each step involved in the construction of the proposed immunosensor. The results showed the potentiality of the device for the quantitative detection of Hantavirus Araucaria nucleoprotein (Np) from 30 mug mL(-1) to 240 mug mL(-1) with a limit of detection of 22 mug mL(-1). Also, the proposed immunosensor was applied with success for virus detection in 100x diluted human serum samples. Therefore, the PLA conductive filament with carbon black is a simple and excellent platform for immunosensing, which offers naturally carboxylic groups able to anchor covalently biomolecules. Twit Text FOAVip 3D-printed electrode as a new platform for electrochemical immunosensors for virus detection ????Anal Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=33485583 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33485583 #FOAvip English Wikipedia <ref>{{Cite pmid|33485583}}</ref> 3D-printed electrode as a new platform for electrochemical immunosensors for virus detection #MMPMID33485583 Martins G; Gogola JL; Budni LH; Janegitz BC; Marcolino-Junior LH; Bergamini MF Anal Chim Acta 2021[Feb]; 1147 (ä): 30-37 PMID33485583show ga Simple, low-cost, and sensitive new platforms for electrochemical immunosensors for virus detection have been attracted attention due to the recent pandemic caused by a new type of coronavirus (SARS-CoV-2). In the present work, we report for the first time the construction of an immunosensor using a commercial 3D conductive filament of carbon black and polylactic acid (PLA) to detect Hantavirus Araucaria nucleoprotein (Np) as a proof-of-concept. The recognition biomolecule was anchored directly at the filament surface by using N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-Hydroxysuccinimide (EDC/NHS). Conductive and non-conductive composites of PLA were characterized using thermal gravimetric analysis (TGA), revealing around 30% w/w of carbon in the filament. Morphological features of composites were obtained from SEM and TEM measurements. FTIR measurement revealed that crosslinking agents were covalently bonded at the filament surface. Electrochemical techniques such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used for the evaluation of each step involved in the construction of the proposed immunosensor. The results showed the potentiality of the device for the quantitative detection of Hantavirus Araucaria nucleoprotein (Np) from 30 mug mL(-1) to 240 mug mL(-1) with a limit of detection of 22 mug mL(-1). Also, the proposed immunosensor was applied with success for virus detection in 100x diluted human serum samples. Therefore, the PLA conductive filament with carbon black is a simple and excellent platform for immunosensing, which offers naturally carboxylic groups able to anchor covalently biomolecules. |*Printing, Three-Dimensional[MESH] |Antibodies, Immobilized/chemistry/immunology[MESH] |Antibodies, Viral/*immunology[MESH] |COVID-19/diagnosis/virology[MESH] |Dielectric Spectroscopy[MESH] |Electrodes[MESH] |Hantavirus Infections/diagnosis/virology[MESH] |Humans[MESH] |Immunoassay/instrumentation/*methods[MESH] |Limit of Detection[MESH] |Nucleocapsid Proteins/blood/*immunology[MESH] |Orthohantavirus/isolation & purification/metabolism[MESH] |SARS-CoV-2/isolation & purification[MESH]3D-printed electrode as a new platform for electrochemical immunosenso(epmc).pdf DeepDyve Pubget Overpricing