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10.1007/s12104-021-10007-w

http://scihub22266oqcxt.onion/10.1007/s12104-021-10007-w
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33484403!7822759!33484403
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suck abstract from ncbi


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pmid33484403      Biomol+NMR+Assign 2021 ; 15 (1): 203-211
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  • (1)H, (13)C and (15)N chemical shift assignment of the stem-loop 5a from the 5 -UTR of SARS-CoV-2 #MMPMID33484403
  • Schnieders R; Peter SA; Banijamali E; Riad M; Altincekic N; Bains JK; Ceylan B; Furtig B; Grun JT; Hengesbach M; Hohmann KF; Hymon D; Knezic B; Oxenfarth A; Petzold K; Qureshi NS; Richter C; Schlagnitweit J; Schlundt A; Schwalbe H; Stirnal E; Sudakov A; Vogele J; Wacker A; Weigand JE; Wirmer-Bartoschek J; Wohnert J
  • Biomol NMR Assign 2021[Apr]; 15 (1): 203-211 PMID33484403show ga
  • The SARS-CoV-2 (SCoV-2) virus is the causative agent of the ongoing COVID-19 pandemic. It contains a positive sense single-stranded RNA genome and belongs to the genus of Betacoronaviruses. The 5'- and 3'-genomic ends of the 30 kb SCoV-2 genome are potential antiviral drug targets. Major parts of these sequences are highly conserved among Betacoronaviruses and contain cis-acting RNA elements that affect RNA translation and replication. The 31 nucleotide (nt) long highly conserved stem-loop 5a (SL5a) is located within the 5'-untranslated region (5'-UTR) important for viral replication. SL5a features a U-rich asymmetric bulge and is capped with a 5'-UUUCGU-3' hexaloop, which is also found in stem-loop 5b (SL5b). We herein report the extensive (1)H, (13)C and (15)N resonance assignment of SL5a as basis for in-depth structural studies by solution NMR spectroscopy.
  • |*5' Untranslated Regions[MESH]
  • |*Magnetic Resonance Spectroscopy[MESH]
  • |Carbon Isotopes[MESH]
  • |Coronavirus Papain-Like Proteases/*chemistry[MESH]
  • |Genes, Viral[MESH]
  • |Hydrogen[MESH]
  • |Nitrogen Isotopes[MESH]
  • |Protein Binding[MESH]
  • |Protein Domains[MESH]
  • |Protein Structure, Secondary[MESH]


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