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10.1038/s41598-021-81389-z http://scihub22266oqcxt.onion/10.1038/s41598-021-81389-z 33479388!7820401!33479388     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33479388&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 1864 Nephropedia Template TP {{tp|p=33479388|t=2021. Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells |pdf=|usr=}}{{33479388}} gab.com Text Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33479388 #FOAvip The ferret is a key animal model for investigating the pathogenicity and transmissibility of important human viruses, and for the pre-clinical assessment of vaccines. However, relatively little is known about the ferret immune system, due in part to a paucity of ferret-reactive reagents. In particular, T follicular helper (Tfh) cells are critical in the generation of effective humoral responses in humans, mice and other animal models but to date it has not been possible to identify Tfh in ferrets. Here, we describe the screening and development of ferret-reactive BCL6, CXCR5 and PD-1 monoclonal antibodies. We found two commercial anti-BCL6 antibodies (clone K112-91 and clone IG191E/A8) had cross-reactivity with lymph node cells from influenza-infected ferrets. We next developed two murine monoclonal antibodies against ferret CXCR5 (clone feX5-C05) and PD-1 (clone fePD-CL1) using a single B cell PCR-based method. We were able to clearly identify Tfh cells in lymph nodes from influenza infected ferrets using these antibodies. The development of ferret Tfh marker antibodies and the identification of ferret Tfh cells will assist the evaluation of vaccine-induced Tfh responses in the ferret model and the design of novel vaccines against the infection of influenza and other viruses, including SARS-CoV2. Twit Text FOAVip Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33479388 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33479388 #FOAvip English Wikipedia <ref>{{Cite pmid|33479388}}</ref> Screening and development of monoclonal antibodies for identification of ferret T follicular helper cells #MMPMID33479388 Jiang W; Wong J; Tan HX; Kelly HG; Whitney PG; Barr I; Layton DS; Kent SJ; Wheatley AK; Juno JA Sci Rep 2021[Jan]; 11 (1): 1864 PMID33479388show ga The ferret is a key animal model for investigating the pathogenicity and transmissibility of important human viruses, and for the pre-clinical assessment of vaccines. However, relatively little is known about the ferret immune system, due in part to a paucity of ferret-reactive reagents. In particular, T follicular helper (Tfh) cells are critical in the generation of effective humoral responses in humans, mice and other animal models but to date it has not been possible to identify Tfh in ferrets. Here, we describe the screening and development of ferret-reactive BCL6, CXCR5 and PD-1 monoclonal antibodies. We found two commercial anti-BCL6 antibodies (clone K112-91 and clone IG191E/A8) had cross-reactivity with lymph node cells from influenza-infected ferrets. We next developed two murine monoclonal antibodies against ferret CXCR5 (clone feX5-C05) and PD-1 (clone fePD-CL1) using a single B cell PCR-based method. We were able to clearly identify Tfh cells in lymph nodes from influenza infected ferrets using these antibodies. The development of ferret Tfh marker antibodies and the identification of ferret Tfh cells will assist the evaluation of vaccine-induced Tfh responses in the ferret model and the design of novel vaccines against the infection of influenza and other viruses, including SARS-CoV2. |Animals[MESH] |Antibodies, Monoclonal/*immunology/isolation & purification[MESH] |COVID-19 Vaccines/immunology[MESH] |Cross Reactions/immunology[MESH] |Ferrets/*immunology[MESH] |High-Throughput Screening Assays/*methods[MESH] |Humans[MESH] |Influenza Vaccines/immunology[MESH] |Lymph Nodes/immunology[MESH] |Mice[MESH] |Programmed Cell Death 1 Receptor/immunology[MESH] |Proto-Oncogene Proteins c-bcl-6/immunology[MESH] |Receptors, CXCR5/immunology[MESH] |T Follicular Helper Cells/*immunology[MESH]Screening and development of monoclonal antibodies for identification (epmc).pdf DeepDyve Pubget Overpricing