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10.1128/JCM.02402-20 http://scihub22266oqcxt.onion/10.1128/JCM.02402-20 33478979!8092748!33478979     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Microbiol 2021 ; 59 (4): ä Nephropedia Template TP {{tp|p=33478979|t=2021. A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material |pdf=|usr=}}{{33478979}} gab.com Text A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material JO: J Clin Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33478979 #FOAvip The COVID-19 pandemic has created massive demand for widespread, distributed tools for detecting SARS-CoV-2 genetic material. The hurdles to scalable testing include reagent and instrument accessibility, availability of highly trained personnel, and large upfront investment. Here, we showcase an orthogonal pipeline we call CREST (Cas13-based, rugged, equitable, scalable testing) that addresses some of these hurdles. Specifically, CREST pairs commonplace and reliable biochemical methods (PCR) with low-cost instrumentation, without sacrificing detection sensitivity. By taking advantage of simple fluorescence visualizers, CREST allows a binary interpretation of results. CREST may provide a point-of-care solution to increase the distribution of COVID-19 surveillance. Twit Text FOAVip A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material ????J Clin Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=33478979 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33478979 #FOAvip English Wikipedia <ref>{{Cite pmid|33478979}}</ref> A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material #MMPMID33478979 Rauch JN; Valois E; Solley SC; Braig F; Lach RS; Audouard M; Ponce-Rojas JC; Costello MS; Baxter NJ; Kosik KS; Arias C; Acosta-Alvear D; Wilson MZ J Clin Microbiol 2021[Mar]; 59 (4): ä PMID33478979show ga The COVID-19 pandemic has created massive demand for widespread, distributed tools for detecting SARS-CoV-2 genetic material. The hurdles to scalable testing include reagent and instrument accessibility, availability of highly trained personnel, and large upfront investment. Here, we showcase an orthogonal pipeline we call CREST (Cas13-based, rugged, equitable, scalable testing) that addresses some of these hurdles. Specifically, CREST pairs commonplace and reliable biochemical methods (PCR) with low-cost instrumentation, without sacrificing detection sensitivity. By taking advantage of simple fluorescence visualizers, CREST allows a binary interpretation of results. CREST may provide a point-of-care solution to increase the distribution of COVID-19 surveillance. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |Humans[MESH] |Pandemics[MESH] |Point-of-Care Systems[MESH]A Scalable, Easy-to-Deploy Protocol for Cas13-Based Detection of SARS-(epmc).pdf DeepDyve Pubget Overpricing