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Deprecated: Implicit conversion from float 296.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Euro+Surveill 2021 ; 26 (3): ä Nephropedia Template TP
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Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69-V70, France, August to December 2020 #MMPMID33478625
Bal A; Destras G; Gaymard A; Stefic K; Marlet J; Eymieux S; Regue H; Semanas Q; d'Aubarede C; Billaud G; Laurent F; Gonzalez C; Mekki Y; Valette M; Bouscambert M; Gaudy-Graffin C; Lina B; Morfin F; Josset L
Euro Surveill 2021[Jan]; 26 (3): ä PMID33478625show ga
We report the strategy leading to the first detection of variant of concern 202012/01 (VOC) in France (21 December 2020). First, the spike (S) deletion H69-V70 (DeltaH69/DeltaV70), identified in certain SARS-CoV-2 variants including VOC, is screened for. This deletion is associated with a S-gene target failure (SGTF) in the three-target RT-PCR assay (TaqPath kit). Subsequently, SGTF samples are whole genome sequenced. This approach revealed mutations co-occurring with DeltaH69/DeltaV70 including S:N501Y in the VOC.