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10.1080/14737159.2021.1873769 http://scihub22266oqcxt.onion/10.1080/14737159.2021.1873769 33474990!ä!33474990 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Expert+Rev+Mol+Diagn 2021 ; 21 (1): 43-61 Nephropedia Template TP {{tp|p=33474990|t=2021. LAMP Diagnostics at the Point-of-Care: Emerging Trends and Perspectives for the Developer Community |pdf=|usr=}}{{33474990}} gab.com Text LAMP Diagnostics at the Point-of-Care: Emerging Trends and Perspectives for the Developer Community JO: Expert Rev Mol Diagn http://www.kidney.de/pget.php?&tw=1&pmid=33474990 #FOAvip Introduction: Over the past decade, loop-mediated isothermal amplification (LAMP) technology has played an important role in molecular diagnostics. Amongst numerous nucleic acid amplification assays, LAMP stands out in terms of sample-to-answer time, sensitivity, specificity, cost, robustness, and accessibility, making it ideal for field-deployable diagnostics in resource-limited regions.Areas covered: In this review, we outline the front-end LAMP design practices for point-of-care (POC) applications, including sample handling and various signal readout methodologies. Next, we explore existing LAMP technologies that have been validated with clinical samples in the field. We summarize recent work that utilizes reverse transcription (RT) LAMP to rapidly detect SARS-CoV-2 as an alternative to standard PCR protocols. Finally, we describe challenges in translating LAMP from the benchtop to the field and opportunities for future LAMP assay development and performance reporting.Expert opinion: Despite the popularity of LAMP in the academic research community and a recent surge in interest in LAMP due to the COVID-19 pandemic, there are numerous areas for improvement in the fundamental understanding of LAMP, which are needed to elevate the field of LAMP assay development and characterization. Twit Text FOAVip LAMP Diagnostics at the Point-of-Care: Emerging Trends and Perspectives for the Developer Community ????Expert Rev Mol Diagn http://www.kidney.de/pget.php?&tw=1&pmid=33474990 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33474990 #FOAvip English Wikipedia <ref>{{Cite pmid|33474990}}</ref> LAMP Diagnostics at the Point-of-Care: Emerging Trends and Perspectives for the Developer Community #MMPMID33474990 Moehling TJ; Choi G; Dugan LC; Salit M; Meagher RJ Expert Rev Mol Diagn 2021[Jan]; 21 (1): 43-61 PMID33474990show ga Introduction: Over the past decade, loop-mediated isothermal amplification (LAMP) technology has played an important role in molecular diagnostics. Amongst numerous nucleic acid amplification assays, LAMP stands out in terms of sample-to-answer time, sensitivity, specificity, cost, robustness, and accessibility, making it ideal for field-deployable diagnostics in resource-limited regions.Areas covered: In this review, we outline the front-end LAMP design practices for point-of-care (POC) applications, including sample handling and various signal readout methodologies. Next, we explore existing LAMP technologies that have been validated with clinical samples in the field. We summarize recent work that utilizes reverse transcription (RT) LAMP to rapidly detect SARS-CoV-2 as an alternative to standard PCR protocols. Finally, we describe challenges in translating LAMP from the benchtop to the field and opportunities for future LAMP assay development and performance reporting.Expert opinion: Despite the popularity of LAMP in the academic research community and a recent surge in interest in LAMP due to the COVID-19 pandemic, there are numerous areas for improvement in the fundamental understanding of LAMP, which are needed to elevate the field of LAMP assay development and characterization. |*COVID-19 Testing[MESH] |*Molecular Diagnostic Techniques[MESH] |*Nucleic Acid Amplification Techniques[MESH] |*Point-of-Care Testing[MESH] |COVID-19/*diagnosis[MESH] |Colorimetry[MESH] |False Positive Reactions[MESH] |Fluorescence[MESH] |Humans[MESH] |Limit of Detection[MESH] |Pandemics[MESH] |Point-of-Care Systems[MESH] |Polymerase Chain Reaction/methods[MESH] |RNA, Viral/isolation & purification[MESH] |SARS-CoV-2/*isolation & purification[MESH]LAMP Diagnostics at the Point-of-Care: Emerging Trends and Perspective(epmc).pdf DeepDyve Pubget Overpricing