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10.1038/s41422-020-00455-9 http://scihub22266oqcxt.onion/10.1038/s41422-020-00455-9 33473155!8027624!33473155     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cell+Res 2021 ; 31 (3): 272-290 Nephropedia Template TP {{tp|p=33473155|t=2021. Discriminating mild from critical COVID-19 by innate and adaptive immune single-cell profiling of bronchoalveolar lavages |pdf=|usr=}}{{33473155}} gab.com Text Discriminating mild from critical COVID-19 by innate and adaptive immune single-cell profiling of bronchoalveolar lavages JO: Cell Res http://www.kidney.de/pget.php?&tw=1&pmid=33473155 #FOAvip How the innate and adaptive host immune system miscommunicate to worsen COVID-19 immunopathology has not been fully elucidated. Here, we perform single-cell deep-immune profiling of bronchoalveolar lavage (BAL) samples from 5 patients with mild and 26 with critical COVID-19 in comparison to BALs from non-COVID-19 pneumonia and normal lung. We use pseudotime inference to build T-cell and monocyte-to-macrophage trajectories and model gene expression changes along them. In mild COVID-19, CD8(+) resident-memory (T(RM)) and CD4(+) T-helper-17 (T(H17)) cells undergo active (presumably antigen-driven) expansion towards the end of the trajectory, and are characterized by good effector functions, while in critical COVID-19 they remain more naive. Vice versa, CD4(+) T-cells with T-helper-1 characteristics (T(H1)-like) and CD8(+) T-cells expressing exhaustion markers (T(EX)-like) are enriched halfway their trajectories in mild COVID-19, where they also exhibit good effector functions, while in critical COVID-19 they show evidence of inflammation-associated stress at the end of their trajectories. Monocyte-to-macrophage trajectories show that chronic hyperinflammatory monocytes are enriched in critical COVID-19, while alveolar macrophages, otherwise characterized by anti-inflammatory and antigen-presenting characteristics, are depleted. In critical COVID-19, monocytes contribute to an ATP-purinergic signaling-inflammasome footprint that could enable COVID-19 associated fibrosis and worsen disease-severity. Finally, viral RNA-tracking reveals infected lung epithelial cells, and a significant proportion of neutrophils and macrophages that are involved in viral clearance. Twit Text FOAVip Discriminating mild from critical COVID-19 by innate and adaptive immune single-cell profiling of bronchoalveolar lavages ????Cell Res http://www.kidney.de/pget.php?&tw=1&pmid=33473155 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33473155 #FOAvip English Wikipedia <ref>{{Cite pmid|33473155}}</ref> Discriminating mild from critical COVID-19 by innate and adaptive immune single-cell profiling of bronchoalveolar lavages #MMPMID33473155 Wauters E; Van Mol P; Garg AD; Jansen S; Van Herck Y; Vanderbeke L; Bassez A; Boeckx B; Malengier-Devlies B; Timmerman A; Van Brussel T; Van Buyten T; Schepers R; Heylen E; Dauwe D; Dooms C; Gunst J; Hermans G; Meersseman P; Testelmans D; Yserbyt J; Tejpar S; De Wever W; Matthys P; Neyts J; Wauters J; Qian J; Lambrechts D Cell Res 2021[Mar]; 31 (3): 272-290 PMID33473155show ga How the innate and adaptive host immune system miscommunicate to worsen COVID-19 immunopathology has not been fully elucidated. Here, we perform single-cell deep-immune profiling of bronchoalveolar lavage (BAL) samples from 5 patients with mild and 26 with critical COVID-19 in comparison to BALs from non-COVID-19 pneumonia and normal lung. We use pseudotime inference to build T-cell and monocyte-to-macrophage trajectories and model gene expression changes along them. In mild COVID-19, CD8(+) resident-memory (T(RM)) and CD4(+) T-helper-17 (T(H17)) cells undergo active (presumably antigen-driven) expansion towards the end of the trajectory, and are characterized by good effector functions, while in critical COVID-19 they remain more naive. Vice versa, CD4(+) T-cells with T-helper-1 characteristics (T(H1)-like) and CD8(+) T-cells expressing exhaustion markers (T(EX)-like) are enriched halfway their trajectories in mild COVID-19, where they also exhibit good effector functions, while in critical COVID-19 they show evidence of inflammation-associated stress at the end of their trajectories. Monocyte-to-macrophage trajectories show that chronic hyperinflammatory monocytes are enriched in critical COVID-19, while alveolar macrophages, otherwise characterized by anti-inflammatory and antigen-presenting characteristics, are depleted. In critical COVID-19, monocytes contribute to an ATP-purinergic signaling-inflammasome footprint that could enable COVID-19 associated fibrosis and worsen disease-severity. Finally, viral RNA-tracking reveals infected lung epithelial cells, and a significant proportion of neutrophils and macrophages that are involved in viral clearance. |*Adaptive Immunity[MESH] |*Bronchoalveolar Lavage[MESH] |*Immunity, Innate[MESH] |*Single-Cell Analysis[MESH] |Bronchoalveolar Lavage Fluid[MESH] |CD4-Positive T-Lymphocytes/cytology[MESH] |CD8-Positive T-Lymphocytes/cytology[MESH] |COVID-19/*diagnosis/*immunology[MESH] |Cell Communication[MESH] |Gene Expression Profiling[MESH] |Humans[MESH] |Lung/virology[MESH] |Macrophages, Alveolar/cytology[MESH] |Monocytes/cytology[MESH] |Neutrophils/cytology[MESH] |Phenotype[MESH] |Principal Component Analysis[MESH] |RNA-Seq[MESH]Discriminating mild from critical COVID-19 by innate and adaptive immu(epmc).pdf DeepDyve Pubget Overpricing