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10.15252/emmm.202013549 http://scihub22266oqcxt.onion/10.15252/emmm.202013549 33471406!7933943!33471406     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       EMBO+Mol+Med 2021 ; 13 (3): e13549 Nephropedia Template TP {{tp|p=33471406|t=2021. Flow cytometry multiplexed method for the detection of neutralizing human antibodies to the native SARS-CoV-2 spike protein |pdf=|usr=}}{{33471406}} gab.com Text Flow cytometry multiplexed method for the detection of neutralizing human antibodies to the native SARS-CoV-2 spike protein JO: EMBO Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=33471406 #FOAvip A correct identification of seropositive individuals for the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection is of paramount relevance to assess the degree of protection of a human population to present and future outbreaks of the COVID-19 pandemic. We describe here a sensitive and quantitative flow cytometry method using the cytometer-friendly non-adherent Jurkat T-cell line that stably expresses the full-length native spike "S" protein of SARS-CoV-2 and a truncated form of the human EGFR that serves a normalizing role. S protein and huEGFRt coding sequences are separated by a T2A self-cleaving sequence, allowing to accurately quantify the presence of anti-S immunoglobulins by calculating a score based on the ratio of fluorescence intensities obtained by double-staining with the test sera and anti-EGFR. The method allows to detect immune individuals regardless of the result of other serological tests or even repeated PCR monitoring. As examples of its use, we show that as much as 28% of the personnel working at the CBMSO in Madrid is already immune. Additionally, we show that anti-S antibodies with protective neutralizing activity are long-lasting and can be detected in sera 8 months after infection. Twit Text FOAVip Flow cytometry multiplexed method for the detection of neutralizing human antibodies to the native SARS-CoV-2 spike protein ????EMBO Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=33471406 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33471406 #FOAvip English Wikipedia <ref>{{Cite pmid|33471406}}</ref> Flow cytometry multiplexed method for the detection of neutralizing human antibodies to the native SARS-CoV-2 spike protein #MMPMID33471406 Horndler L; Delgado P; Abia D; Balabanov I; Martinez-Fleta P; Cornish G; Llamas MA; Serrano-Villar S; Sanchez-Madrid F; Fresno M; van Santen HM; Alarcon B EMBO Mol Med 2021[Mar]; 13 (3): e13549 PMID33471406show ga A correct identification of seropositive individuals for the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection is of paramount relevance to assess the degree of protection of a human population to present and future outbreaks of the COVID-19 pandemic. We describe here a sensitive and quantitative flow cytometry method using the cytometer-friendly non-adherent Jurkat T-cell line that stably expresses the full-length native spike "S" protein of SARS-CoV-2 and a truncated form of the human EGFR that serves a normalizing role. S protein and huEGFRt coding sequences are separated by a T2A self-cleaving sequence, allowing to accurately quantify the presence of anti-S immunoglobulins by calculating a score based on the ratio of fluorescence intensities obtained by double-staining with the test sera and anti-EGFR. The method allows to detect immune individuals regardless of the result of other serological tests or even repeated PCR monitoring. As examples of its use, we show that as much as 28% of the personnel working at the CBMSO in Madrid is already immune. Additionally, we show that anti-S antibodies with protective neutralizing activity are long-lasting and can be detected in sera 8 months after infection. |Adult[MESH] |Antibodies, Neutralizing/*blood[MESH] |Antibodies, Viral/*blood[MESH] |COVID-19 Serological Testing/*methods/statistics & numerical data[MESH] |COVID-19/*diagnosis/*immunology/virology[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |ErbB Receptors/genetics[MESH] |Female[MESH] |Flow Cytometry/*methods/statistics & numerical data[MESH] |Hep G2 Cells[MESH] |Humans[MESH] |Jurkat Cells[MESH] |Male[MESH] |Middle Aged[MESH] |Neutralization Tests[MESH] |Pandemics[MESH] |Polymerase Chain Reaction[MESH] |Recombinant Fusion Proteins/genetics/immunology[MESH] |SARS-CoV-2/genetics/*immunology[MESH]Flow cytometry multiplexed method for the detection of neutralizing hu(epmc).pdf DeepDyve Pubget Overpricing