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10.1038/s41598-020-80352-8 http://scihub22266oqcxt.onion/10.1038/s41598-020-80352-8 33469065!7815738!33469065     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=33469065&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 1820 Nephropedia Template TP {{tp|p=33469065|t=2021. Direct detection of SARS-CoV-2 using non-commercial RT-LAMP reagents on heat-inactivated samples |pdf=|usr=}}{{33469065}} gab.com Text Direct detection of SARS-CoV-2 using non-commercial RT-LAMP reagents on heat-inactivated samples JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33469065 #FOAvip RT-LAMP detection of SARS-CoV-2 has been shown to be a valuable approach to scale up COVID-19 diagnostics and thus contribute to limiting the spread of the disease. Here we present the optimization of highly cost-effective in-house produced enzymes, and we benchmark their performance against commercial alternatives. We explore the compatibility between multiple DNA polymerases with high strand-displacement activity and thermostable reverse transcriptases required for RT-LAMP. We optimize reaction conditions and demonstrate their applicability using both synthetic RNA and clinical patient samples. Finally, we validate the optimized RT-LAMP assay for the detection of SARS-CoV-2 in unextracted heat-inactivated nasopharyngeal samples from 184 patients. We anticipate that optimized and affordable reagents for RT-LAMP will facilitate the expansion of SARS-CoV-2 testing globally, especially in sites and settings where the need for large scale testing cannot be met by commercial alternatives. Twit Text FOAVip Direct detection of SARS-CoV-2 using non-commercial RT-LAMP reagents on heat-inactivated samples ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=33469065 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33469065 #FOAvip English Wikipedia <ref>{{Cite pmid|33469065}}</ref> Direct detection of SARS-CoV-2 using non-commercial RT-LAMP reagents on heat-inactivated samples #MMPMID33469065 Alekseenko A; Barrett D; Pareja-Sanchez Y; Howard RJ; Strandback E; Ampah-Korsah H; Rovsnik U; Zuniga-Veliz S; Klenov A; Malloo J; Ye S; Liu X; Reinius B; Elsasser SJ; Nyman T; Sandh G; Yin X; Pelechano V Sci Rep 2021[Jan]; 11 (1): 1820 PMID33469065show ga RT-LAMP detection of SARS-CoV-2 has been shown to be a valuable approach to scale up COVID-19 diagnostics and thus contribute to limiting the spread of the disease. Here we present the optimization of highly cost-effective in-house produced enzymes, and we benchmark their performance against commercial alternatives. We explore the compatibility between multiple DNA polymerases with high strand-displacement activity and thermostable reverse transcriptases required for RT-LAMP. We optimize reaction conditions and demonstrate their applicability using both synthetic RNA and clinical patient samples. Finally, we validate the optimized RT-LAMP assay for the detection of SARS-CoV-2 in unextracted heat-inactivated nasopharyngeal samples from 184 patients. We anticipate that optimized and affordable reagents for RT-LAMP will facilitate the expansion of SARS-CoV-2 testing globally, especially in sites and settings where the need for large scale testing cannot be met by commercial alternatives. |COVID-19/*diagnosis/virology[MESH] |Hot Temperature[MESH] |Humans[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Nasopharynx/virology[MESH] |Nucleic Acid Amplification Techniques/*methods[MESH] |RNA, Viral/metabolism[MESH] |RNA-Directed DNA Polymerase/metabolism[MESH] |Reagent Kits, Diagnostic[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH] |Sensitivity and Specificity[MESH]Direct detection of SARS-CoV-2 using non-commercial RT-LAMP reagents o(epmc).pdf DeepDyve Pubget Overpricing