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10.1371/journal.ppat.1009226 http://scihub22266oqcxt.onion/10.1371/journal.ppat.1009226 33465137!7846108!33465137     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+Pathog 2021 ; 17 (1): e1009226 Nephropedia Template TP {{tp|p=33465137|t=2021. The coronavirus proofreading exoribonuclease mediates extensive viral recombination |pdf=|usr=}}{{33465137}} gab.com Text The coronavirus proofreading exoribonuclease mediates extensive viral recombination JO: PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=33465137 #FOAvip Recombination is proposed to be critical for coronavirus (CoV) diversity and emergence of SARS-CoV-2 and other zoonotic CoVs. While RNA recombination is required during normal CoV replication, the mechanisms and determinants of CoV recombination are not known. CoVs encode an RNA proofreading exoribonuclease (nsp14-ExoN) that is distinct from the CoV polymerase and is responsible for high-fidelity RNA synthesis, resistance to nucleoside analogues, immune evasion, and virulence. Here, we demonstrate that CoVs, including SARS-CoV-2, MERS-CoV, and the model CoV murine hepatitis virus (MHV), generate extensive and diverse recombination products during replication in culture. We show that the MHV nsp14-ExoN is required for native recombination, and that inactivation of ExoN results in decreased recombination frequency and altered recombination products. These results add yet another critical function to nsp14-ExoN, highlight the uniqueness of the evolved coronavirus replicase, and further emphasize nsp14-ExoN as a central, completely conserved, and vulnerable target for inhibitors and attenuation of SARS-CoV-2 and future emerging zoonotic CoVs. Twit Text FOAVip The coronavirus proofreading exoribonuclease mediates extensive viral recombination ????PLoS Pathog http://www.kidney.de/pget.php?&tw=1&pmid=33465137 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33465137 #FOAvip English Wikipedia <ref>{{Cite pmid|33465137}}</ref> The coronavirus proofreading exoribonuclease mediates extensive viral recombination #MMPMID33465137 Gribble J; Stevens LJ; Agostini ML; Anderson-Daniels J; Chappell JD; Lu X; Pruijssers AJ; Routh AL; Denison MR PLoS Pathog 2021[Jan]; 17 (1): e1009226 PMID33465137show ga Recombination is proposed to be critical for coronavirus (CoV) diversity and emergence of SARS-CoV-2 and other zoonotic CoVs. While RNA recombination is required during normal CoV replication, the mechanisms and determinants of CoV recombination are not known. CoVs encode an RNA proofreading exoribonuclease (nsp14-ExoN) that is distinct from the CoV polymerase and is responsible for high-fidelity RNA synthesis, resistance to nucleoside analogues, immune evasion, and virulence. Here, we demonstrate that CoVs, including SARS-CoV-2, MERS-CoV, and the model CoV murine hepatitis virus (MHV), generate extensive and diverse recombination products during replication in culture. We show that the MHV nsp14-ExoN is required for native recombination, and that inactivation of ExoN results in decreased recombination frequency and altered recombination products. These results add yet another critical function to nsp14-ExoN, highlight the uniqueness of the evolved coronavirus replicase, and further emphasize nsp14-ExoN as a central, completely conserved, and vulnerable target for inhibitors and attenuation of SARS-CoV-2 and future emerging zoonotic CoVs. |*COVID-19 Drug Treatment[MESH] |Antiviral Agents/pharmacology[MESH] |COVID-19/virology[MESH] |Coronavirus Infections/*drug therapy/virology[MESH] |Exoribonucleases/genetics/*pharmacology[MESH] |Humans[MESH] |Recombination, Genetic/drug effects[MESH] |SARS-CoV-2/*drug effects/pathogenicity[MESH] |Viral Nonstructural Proteins/genetics[MESH]The coronavirus proofreading exoribonuclease mediates extensive viral (epmc).pdf DeepDyve Pubget Overpricing