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10.1016/j.jviromet.2021.114068 http://scihub22266oqcxt.onion/10.1016/j.jviromet.2021.114068 33460683!ä!33460683 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Virol+Methods 2021 ; 290 (ä): 114068 Nephropedia Template TP {{tp|p=33460683|t=2021. A potential system for the isolation and propagation of porcine deltacoronavirus using embryonated chicken eggs |pdf=|usr=}}{{33460683}} gab.com Text A potential system for the isolation and propagation of porcine deltacoronavirus using embryonated chicken eggs JO: J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33460683 #FOAvip Porcine deltacoronavirus (PDCoV) is a novel swine enteropathogenic coronavirus that leads to acute diarrhea/vomiting, dehydration, and mortality in seronegative neonatal piglets. As widely known, attempts to culture porcine enteropathogenic coronaviruses, such as PDCoV and porcine epidemic diarrhea virus, in cells have been proven to be difficult. This study aimed to establish an efficient and cost-effective culture system for PDCoV using embryonated chicken eggs (ECEs) to enable future vaccine production and efficient virus isolation from infected animals. The inoculation of samples into the allantoic cavity of 3- to 7-day-old ECEs yielded efficient virus propagation even from porcine fecal samples. Virus propagation in 2- and 8-day-old ECEs were confirmed in 30.0 % and 11.1 % of the samples, respectively. This indicates that susceptible cells rapidly develop in 2-day-old ECEs and differentiate to mature cells that are nonsusceptible to PDCoV in 8-day-old layer chicken ECEs. Furthermore, our study demonstrated that PDCoV can be passaged in 6-day-old ECEs with high viral replicative efficiency. This technique for propagating PDCoV using ECEs is a powerful tool that could be utilized for PDCoV vaccine development and virus isolation from poultry, livestock, and wild animals. Twit Text FOAVip A potential system for the isolation and propagation of porcine deltacoronavirus using embryonated chicken eggs ????J Virol Methods http://www.kidney.de/pget.php?&tw=1&pmid=33460683 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33460683 #FOAvip English Wikipedia <ref>{{Cite pmid|33460683}}</ref> A potential system for the isolation and propagation of porcine deltacoronavirus using embryonated chicken eggs #MMPMID33460683 Iseki H; Watanabe S; Mase M J Virol Methods 2021[Apr]; 290 (ä): 114068 PMID33460683show ga Porcine deltacoronavirus (PDCoV) is a novel swine enteropathogenic coronavirus that leads to acute diarrhea/vomiting, dehydration, and mortality in seronegative neonatal piglets. As widely known, attempts to culture porcine enteropathogenic coronaviruses, such as PDCoV and porcine epidemic diarrhea virus, in cells have been proven to be difficult. This study aimed to establish an efficient and cost-effective culture system for PDCoV using embryonated chicken eggs (ECEs) to enable future vaccine production and efficient virus isolation from infected animals. The inoculation of samples into the allantoic cavity of 3- to 7-day-old ECEs yielded efficient virus propagation even from porcine fecal samples. Virus propagation in 2- and 8-day-old ECEs were confirmed in 30.0 % and 11.1 % of the samples, respectively. This indicates that susceptible cells rapidly develop in 2-day-old ECEs and differentiate to mature cells that are nonsusceptible to PDCoV in 8-day-old layer chicken ECEs. Furthermore, our study demonstrated that PDCoV can be passaged in 6-day-old ECEs with high viral replicative efficiency. This technique for propagating PDCoV using ECEs is a powerful tool that could be utilized for PDCoV vaccine development and virus isolation from poultry, livestock, and wild animals. |Amniotic Fluid/virology[MESH] |Animals[MESH] |Chick Embryo[MESH] |Coronavirus Infections/veterinary/virology[MESH] |Deltacoronavirus/*growth & development/*isolation & purification[MESH] |Serial Passage[MESH] |Swine[MESH] |Swine Diseases/virology[MESH] |Virus Cultivation/*methods[MESH]A potential system for the isolation and propagation of porcine deltac(epmc).pdf DeepDyve Pubget Overpricing