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10.1371/journal.pone.0243712 http://scihub22266oqcxt.onion/10.1371/journal.pone.0243712 33428641!7799764!33428641     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2021 ; 16 (1): e0243712 Nephropedia Template TP {{tp|p=33428641|t=2021. Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification |pdf=|usr=}}{{33428641}} gab.com Text Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33428641 #FOAvip To respond to the urgent need for COVID-19 testing, countries perform nucleic acid amplification tests (NAAT) for the detection of SARS-CoV-2 in centralized laboratories. Real-time RT-PCR (Reverse transcription-Polymerase Chain Reaction), used to amplify and detect the viral RNA., is considered, as the current gold standard for diagnostics. It is an efficient process, but the complex engineering required for automated RNA extraction and temperature cycling makes it incompatible for use in point of care settings [1]. In the present work, by harnessing progress made in the past two decades in isothermal amplification and paper microfluidics, we created a portable test, in which SARS-CoV-2 RNA is extracted, amplified isothermally by RT-LAMP (Loop-mediated Isothermal Amplification), and detected using intercalating dyes or fluorescent probes. Depending on the viral load in the tested samples, the detection takes between twenty minutes and one hour. Using a set of 16 pools of naso-pharyngal swab eluates, we estimated a limit of detection comparable to real-time RT-PCR (i.e. 1 genome copies per microliter of clinical sample) and no cross-reaction with eight major respiratory viruses currently circulating in Europe. We designed and fabricated an easy-to-use portable device called "COVIDISC" to carry out the test at the point of care. The low cost of the materials along with the absence of complex equipment will expedite the widespread dissemination of this device. What is proposed here is a new efficient tool to help managing the pandemics. Twit Text FOAVip Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=33428641 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33428641 #FOAvip English Wikipedia <ref>{{Cite pmid|33428641}}</ref> Performing point-of-care molecular testing for SARS-CoV-2 with RNA extraction and isothermal amplification #MMPMID33428641 Garneret P; Coz E; Martin E; Manuguerra JC; Brient-Litzler E; Enouf V; Gonzalez Obando DF; Olivo-Marin JC; Monti F; van der Werf S; Vanhomwegen J; Tabeling P PLoS One 2021[]; 16 (1): e0243712 PMID33428641show ga To respond to the urgent need for COVID-19 testing, countries perform nucleic acid amplification tests (NAAT) for the detection of SARS-CoV-2 in centralized laboratories. Real-time RT-PCR (Reverse transcription-Polymerase Chain Reaction), used to amplify and detect the viral RNA., is considered, as the current gold standard for diagnostics. It is an efficient process, but the complex engineering required for automated RNA extraction and temperature cycling makes it incompatible for use in point of care settings [1]. In the present work, by harnessing progress made in the past two decades in isothermal amplification and paper microfluidics, we created a portable test, in which SARS-CoV-2 RNA is extracted, amplified isothermally by RT-LAMP (Loop-mediated Isothermal Amplification), and detected using intercalating dyes or fluorescent probes. Depending on the viral load in the tested samples, the detection takes between twenty minutes and one hour. Using a set of 16 pools of naso-pharyngal swab eluates, we estimated a limit of detection comparable to real-time RT-PCR (i.e. 1 genome copies per microliter of clinical sample) and no cross-reaction with eight major respiratory viruses currently circulating in Europe. We designed and fabricated an easy-to-use portable device called "COVIDISC" to carry out the test at the point of care. The low cost of the materials along with the absence of complex equipment will expedite the widespread dissemination of this device. What is proposed here is a new efficient tool to help managing the pandemics. |*Point-of-Care Testing/economics[MESH] |COVID-19 Testing/economics/*instrumentation[MESH] |COVID-19/*diagnosis[MESH] |Equipment Design[MESH] |Humans[MESH] |Limit of Detection[MESH] |Molecular Diagnostic Techniques/economics/*instrumentation[MESH] |Nucleic Acid Amplification Techniques/economics/*instrumentation[MESH] |RNA, Viral/*genetics/isolation & purification[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH]Performing point-of-care molecular testing for SARS-CoV-2 with RNA ext(epmc).pdf DeepDyve Pubget Overpricing