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10.1177/2472555220985040 http://scihub22266oqcxt.onion/10.1177/2472555220985040 33423577!7803792!33423577     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       SLAS+Discov 2021 ; 26 (5): 620-627 Nephropedia Template TP {{tp|p=33423577|t=2021. A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19 |pdf=|usr=}}{{33423577}} gab.com Text A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19 JO: SLAS Discov http://www.kidney.de/pget.php?&tw=1&pmid=33423577 #FOAvip SARS-CoV-2, the coronavirus that causes COVID-19, evades the human immune system by capping its RNA. This process protects the viral RNA and is essential for its replication. Multiple viral proteins are involved in this RNA capping process, including the nonstructural protein 16 (nsp16), which is an S-adenosyl-l-methionine (SAM)-dependent 2'-O-methyltransferase. Nsp16 is significantly active when in complex with another nonstructural protein, nsp10, which plays a key role in its stability and activity. Here we report the development of a fluorescence polarization (FP)-based RNA displacement assay for nsp10-nsp16 complex in a 384-well format with a Z' factor of 0.6, suitable for high-throughput screening. In this process, we purified the nsp10-nsp16 complex to higher than 95% purity and confirmed its binding to the methyl donor SAM, the product of the reaction, S-adenosyl-l-homocysteine (SAH), and a common methyltransferase inhibitor, sinefungin, using isothermal titration calorimetry (ITC). The assay was further validated by screening a library of 1124 drug-like compounds. This assay provides a cost-effective high-throughput method for screening the nsp10-nsp16 complex for RNA competitive inhibitors toward developing COVID-19 therapeutics. Twit Text FOAVip A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19 ????SLAS Discov http://www.kidney.de/pget.php?&tw=1&pmid=33423577 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33423577 #FOAvip English Wikipedia <ref>{{Cite pmid|33423577}}</ref> A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19 #MMPMID33423577 Perveen S; Khalili Yazdi A; Devkota K; Li F; Ghiabi P; Hajian T; Loppnau P; Bolotokova A; Vedadi M SLAS Discov 2021[Jun]; 26 (5): 620-627 PMID33423577show ga SARS-CoV-2, the coronavirus that causes COVID-19, evades the human immune system by capping its RNA. This process protects the viral RNA and is essential for its replication. Multiple viral proteins are involved in this RNA capping process, including the nonstructural protein 16 (nsp16), which is an S-adenosyl-l-methionine (SAM)-dependent 2'-O-methyltransferase. Nsp16 is significantly active when in complex with another nonstructural protein, nsp10, which plays a key role in its stability and activity. Here we report the development of a fluorescence polarization (FP)-based RNA displacement assay for nsp10-nsp16 complex in a 384-well format with a Z' factor of 0.6, suitable for high-throughput screening. In this process, we purified the nsp10-nsp16 complex to higher than 95% purity and confirmed its binding to the methyl donor SAM, the product of the reaction, S-adenosyl-l-homocysteine (SAH), and a common methyltransferase inhibitor, sinefungin, using isothermal titration calorimetry (ITC). The assay was further validated by screening a library of 1124 drug-like compounds. This assay provides a cost-effective high-throughput method for screening the nsp10-nsp16 complex for RNA competitive inhibitors toward developing COVID-19 therapeutics. |*High-Throughput Screening Assays[MESH] |Adenosine/analogs & derivatives/pharmacology[MESH] |Antiviral Agents/*pharmacology[MESH] |Binding, Competitive[MESH] |COVID-19 Drug Treatment[MESH] |COVID-19/virology[MESH] |Enzyme Inhibitors/pharmacology[MESH] |Fluorescence Polarization[MESH] |Gene Expression Regulation[MESH] |Host-Pathogen Interactions/drug effects[MESH] |Humans[MESH] |Methyltransferases[MESH] |Protein Binding[MESH] |RNA Caps/antagonists & inhibitors/genetics/metabolism[MESH] |RNA, Viral/*antagonists & inhibitors/genetics/metabolism[MESH] |SARS-CoV-2/*drug effects/genetics/metabolism[MESH] |Signal Transduction[MESH] |Small Molecule Libraries/*pharmacology[MESH] |Viral Nonstructural Proteins/*antagonists & inhibitors/genetics/metabolism[MESH] |Viral Regulatory and Accessory Proteins/*antagonists & inhibitors/genetics/metabolism[MESH]A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp1(epmc).pdf DeepDyve Pubget Overpricing