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Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Nat+Commun 2021 ; 12 (1): 134 Nephropedia Template TP
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Host and viral determinants for efficient SARS-CoV-2 infection of the human lung #MMPMID33420022
Chu H; Hu B; Huang X; Chai Y; Zhou D; Wang Y; Shuai H; Yang D; Hou Y; Zhang X; Yuen TT; Cai JP; Zhang AJ; Zhou J; Yuan S; To KK; Chan IH; Sit KY; Foo DC; Wong IY; Ng AT; Cheung TT; Law SY; Au WK; Brindley MA; Chen Z; Kok KH; Chan JF; Yuen KY
Nat Commun 2021[Jan]; 12 (1): 134 PMID33420022show ga
Understanding the factors that contribute to efficient SARS-CoV-2 infection of human cells may provide insights on SARS-CoV-2 transmissibility and pathogenesis, and reveal targets of intervention. Here, we analyze host and viral determinants essential for efficient SARS-CoV-2 infection in both human lung epithelial cells and ex vivo human lung tissues. We identify heparan sulfate as an important attachment factor for SARS-CoV-2 infection. Next, we show that sialic acids present on ACE2 prevent efficient spike/ACE2-interaction. While SARS-CoV infection is substantially limited by the sialic acid-mediated restriction in both human lung epithelial cells and ex vivo human lung tissues, infection by SARS-CoV-2 is limited to a lesser extent. We further demonstrate that the furin-like cleavage site in SARS-CoV-2 spike is required for efficient virus replication in human lung but not intestinal tissues. These findings provide insights on the efficient SARS-CoV-2 infection of human lungs.