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10.1016/j.bios.2020.112920

http://scihub22266oqcxt.onion/10.1016/j.bios.2020.112920
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33418184!7834412!33418184
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suck abstract from ncbi


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pmid33418184      Biosens+Bioelectron 2021 ; 176 (ä): 112920
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  • Reciprocating-flowing on-a-chip enables ultra-fast immunobinding for multiplexed rapid ELISA detection of SARS-CoV-2 antibody #MMPMID33418184
  • Liu Y; Tan Y; Fu Q; Lin M; He J; He S; Yang M; Chen S; Zhou J
  • Biosens Bioelectron 2021[Mar]; 176 (ä): 112920 PMID33418184show ga
  • The worldwide epidemic of novel coronavirus disease (COVID-19) has led to a strong demand for highly efficient immunobinding to achieve rapid and accurate on-site detection of SARS-CoV-2 antibodies. However, hour-scale time-consumption is usually required to ensure the adequacy of immunobinding on expensive large instruments in hospitals, and the common false negative or positive results often occur in rapid on-site immunoassay (e.g. immunochromatography). We solved this dilemma by presenting a reciprocating-flowing immunobinding (RF-immunobinding) strategy. RF-immunobinding enabled the antibodies in fluid contacting with the corresponding immobilized antigens on substrate repeatedly during continuous reciprocating-flowing, to achieve adequate immunobinding within 60 s. This strategy was further developed into an immunoassay method for the serological detection of 13 suspected COVID-19 patients. We obtained a 100% true negative and true positive rate and a limit of quantification (LOQ) of 4.14 pg/mL. Our strategy also can be a potential support for other areas related to immunorecognition, such as proteomics, immunopharmacology and immunohistochemistry.
  • |*Lab-On-A-Chip Devices[MESH]
  • |Antibodies, Viral/blood[MESH]
  • |Antigen-Antibody Reactions[MESH]
  • |Biosensing Techniques/instrumentation[MESH]
  • |COVID-19 Serological Testing/*instrumentation/methods[MESH]
  • |COVID-19/*diagnosis/immunology/virology[MESH]
  • |Enzyme-Linked Immunosorbent Assay/instrumentation[MESH]
  • |Equipment Design[MESH]
  • |Humans[MESH]
  • |Immobilized Proteins[MESH]
  • |Pandemics[MESH]


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