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10.1016/j.jcv.2020.104720 http://scihub22266oqcxt.onion/10.1016/j.jcv.2020.104720 33418142!7774005!33418142     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Virol 2021 ; 135 (ä): 104720 Nephropedia Template TP {{tp|p=33418142|t=2021. A two minute liquid based sample preparation for rapid SARS-CoV2 real-time PCR screening: A multicentre evaluation |pdf=|usr=}}{{33418142}} gab.com Text A two minute liquid based sample preparation for rapid SARS-CoV2 real-time PCR screening: A multicentre evaluation JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=33418142 #FOAvip BACKGROUND: Apart from major health concerns associated to the SARS-coronavirus-2 (SARS-CoV-2) pandemic, also the diagnostic workflow encountered serious problems. Limited availability of kit components, buffers and even plastics has resulted in suboptimal testing procedures worldwide. Alternative workflows have been implemented to overcome these difficulties. Recently a liquid based sample prep has been launched as solution to overcome limitations in relation to nucleic acid extraction. OBJECTIVE: Multicenter evaluation of the QIAprep& Viral RNA UM kit (QIA P&A) for rapid sample preparation and real-time PCR detection of SARS-CoV-2 in comparison to standardized laboratory testing methods. STUDY DESIGN: Selected samples of the routine diagnostic workflow at Clinical Microbiology Laboratories of four Dutch hospitals have been subjected to the rapid QIA P&A protocol and the results have been compared to routine diagnostic data. RESULTS: Combining results of manual and automated procedures, a total of 377 clinical samples of which 202 had been tested positive with a wide range of C(T) values, showed almost complete concordance in the QIA P&A assay for samples up to C(T) values of 33 with one exception of C(T) 31. Prospectively 60 samples were tested and also showed 100 % concordance with 5 positives. The method has been automated by two centres. CONCLUSIONS: Despite an input of only 8 muL of clinical sample, the QIA P&A kit showed good performance for sample preparation and amplification of SARS-CoV-2 and can contribute as a rapid molecular testing strategy in managing the CoV-2 pandemic. Twit Text FOAVip A two minute liquid based sample preparation for rapid SARS-CoV2 real-time PCR screening: A multicentre evaluation ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=33418142 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=33418142 #FOAvip English Wikipedia <ref>{{Cite pmid|33418142}}</ref> A two minute liquid based sample preparation for rapid SARS-CoV2 real-time PCR screening: A multicentre evaluation #MMPMID33418142 Claas ECJ; Smit PW; van Bussel MJAWM; Verbakel H; Taouil M; Verweij JJ; Thijsen SFT J Clin Virol 2021[Feb]; 135 (ä): 104720 PMID33418142show ga BACKGROUND: Apart from major health concerns associated to the SARS-coronavirus-2 (SARS-CoV-2) pandemic, also the diagnostic workflow encountered serious problems. Limited availability of kit components, buffers and even plastics has resulted in suboptimal testing procedures worldwide. Alternative workflows have been implemented to overcome these difficulties. Recently a liquid based sample prep has been launched as solution to overcome limitations in relation to nucleic acid extraction. OBJECTIVE: Multicenter evaluation of the QIAprep& Viral RNA UM kit (QIA P&A) for rapid sample preparation and real-time PCR detection of SARS-CoV-2 in comparison to standardized laboratory testing methods. STUDY DESIGN: Selected samples of the routine diagnostic workflow at Clinical Microbiology Laboratories of four Dutch hospitals have been subjected to the rapid QIA P&A protocol and the results have been compared to routine diagnostic data. RESULTS: Combining results of manual and automated procedures, a total of 377 clinical samples of which 202 had been tested positive with a wide range of C(T) values, showed almost complete concordance in the QIA P&A assay for samples up to C(T) values of 33 with one exception of C(T) 31. Prospectively 60 samples were tested and also showed 100 % concordance with 5 positives. The method has been automated by two centres. CONCLUSIONS: Despite an input of only 8 muL of clinical sample, the QIA P&A kit showed good performance for sample preparation and amplification of SARS-CoV-2 and can contribute as a rapid molecular testing strategy in managing the CoV-2 pandemic. |COVID-19 Testing/*methods[MESH] |COVID-19/*diagnosis/*virology[MESH] |Clinical Laboratory Techniques/methods[MESH] |Humans[MESH] |Mass Screening/*methods[MESH] |Molecular Diagnostic Techniques/methods[MESH] |Pandemics/prevention & control[MESH] |Prospective Studies[MESH] |Real-Time Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2/*genetics[MESH] |Specimen Handling/methods[MESH]A two minute liquid based sample preparation for rapid SARS-CoV2 real-(epmc).pdf DeepDyve Pubget Overpricing